Detailed information of TA system TA064576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 972962..973656
• Replicon: chromosome
• Accession: NZ_CP016404
• Organism: Escherichia coli strain 210221272

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: BBP24_RS04790
• Protein ID: WP_001263493.1
• Coordinates: 973258..973656 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: BBP24_RS04785
• Protein ID: WP_000554757.1
• Coordinates: 972962..973255 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBP24_RS04760	968779..969246	+	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-
BBP24_RS04765	969266..969982	+	717	WP_000938723.1	FliA/WhiG family RNA polymerase sigma factor	-
BBP24_RS04770	969995..970858	+	864	WP_001169518.1	flagellar motor stator protein MotA	-
BBP24_RS04775	970861..971784	+	924	WP_001232547.1	putative lateral flagellar export/assembly protein LafU	-
BBP24_RS04780	971855..972910	+	1056	WP_001226164.1	DNA polymerase IV	-
BBP24_RS04785	972962..973255	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
BBP24_RS04790	973258..973656	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
BBP24_RS04795	973666..974118	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
BBP24_RS04800	974364..974567	+	204	Protein_887	RNA ligase RtcB family protein	-
BBP24_RS04805	974566..975087	+	522	Protein_888	peptide chain release factor H	-
BBP24_RS04810	975144..976601	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
BBP24_RS04815	976862..977320	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
BBP24_RS04820	977412..978656	+	1245	WP_000189541.1	esterase FrsA	-
-	977916..977996	+	81	NuclAT_10	-	-
-	977916..977996	+	81	NuclAT_10	-	-
-	977916..977996	+	81	NuclAT_10	-	-
-	977916..977996	+	81	NuclAT_10	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T64576 WP_001263493.1 NZ_CP016404:973258-973656 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T64576 NZ_CP016404:973258-973656 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT64576 WP_000554757.1 NZ_CP016404:972962-973255 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT64576 NZ_CP016404:972962-973255 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6