Detailed information of TA system TA064525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3840859..3841553
• Replicon: chromosome
• Accession: NZ_CP016358
• Organism: Escherichia coli strain K-15KW01

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: BA058_RS18740
• Protein ID: WP_001263500.1
• Coordinates: 3841155..3841553 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: BA058_RS18735
• Protein ID: WP_000554758.1
• Coordinates: 3840859..3841152 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BA058_RS18715	3836395..3836892	+	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-
BA058_RS18720	3837212..3838924	-	1713	Protein_3555	flagellar biosynthesis protein FlhA	-
BA058_RS18725	3838896..3839681	+	786	WP_000207578.1	putative lateral flagellar export/assembly protein LafU	-
BA058_RS18730	3839752..3840807	+	1056	WP_001226168.1	DNA polymerase IV	-
BA058_RS18735	3840859..3841152	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
BA058_RS18740	3841155..3841553	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
BA058_RS18745	3841563..3842015	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
BA058_RS18750	3842205..3843344	+	1140	WP_000521561.1	RNA ligase RtcB family protein	-
BA058_RS18755	3843341..3843955	+	615	WP_000602124.1	peptide chain release factor H	-
BA058_RS18760	3844012..3845469	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
BA058_RS18765	3845730..3846188	+	459	WP_001291991.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T64525 WP_001263500.1 NZ_CP016358:3841155-3841553 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T64525 NZ_CP016358:3841155-3841553 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT64525 WP_000554758.1 NZ_CP016358:3840859-3841152 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT64525 NZ_CP016358:3840859-3841152 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3