Detailed information of TA system TA064514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2799728..2800330
• Replicon: chromosome
• Accession: NZ_CP016358
• Organism: Escherichia coli strain K-15KW01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: BA058_RS13580
• Protein ID: WP_000897305.1
• Coordinates: 2799728..2800039 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BA058_RS13585
• Protein ID: WP_000356397.1
• Coordinates: 2800040..2800330 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BA058_RS13555	2795642..2796241	+	600	WP_001298594.1	glucose-1-phosphatase	-
BA058_RS13560	2796235..2797107	+	873	WP_000920762.1	virulence factor BrkB family protein	-
BA058_RS13565	2797104..2797541	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
BA058_RS13570	2797586..2798527	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
BA058_RS13575	2798591..2799499	-	909	WP_001298596.1	alpha/beta hydrolase	-
BA058_RS13580	2799728..2800039	+	312	WP_000897305.1	hypothetical protein	Toxin
BA058_RS13585	2800040..2800330	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
BA058_RS13595	2800935..2801153	+	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
BA058_RS13600	2801368..2802297	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
BA058_RS13605	2802294..2802929	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
BA058_RS13610	2802926..2803828	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12172.16 Da
• Isoelectric Point: 9.7791

>T64514 WP_000897305.1 NZ_CP016358:2799728-2800039 [Escherichia coli]
ILFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T64514 NZ_CP016358:2799728-2800039 [Escherichia coli]
ATCCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT64514 WP_000356397.1 NZ_CP016358:2800040-2800330 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT64514 NZ_CP016358:2800040-2800330 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7