Detailed information of TA system TA064495

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 683861..684377
• Replicon: chromosome
• Accession: NZ_CP016355
• Organism: Lacticaseibacillus paracasei subsp. paracasei strain TMW 1.1434

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: A0A243Q0H7
• Locus tag: BBD24_RS03430
• Protein ID: WP_003573774.1
• Coordinates: 684096..684377 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: BBD24_RS03425
• Protein ID: WP_016363783.1
• Coordinates: 683861..684103 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBD24_RS03405	679315..681486	-	2172	WP_003607130.1	class 1b ribonucleoside-diphosphate reductase subunit alpha	-
BBD24_RS03410	681658..682647	+	990	WP_003586769.1	class 1b ribonucleoside-diphosphate reductase subunit beta	-
BBD24_RS03415	682644..683129	+	486	WP_003586772.1	class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI	-
BBD24_RS03425	683861..684103	+	243	WP_016363783.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BBD24_RS03430	684096..684377	+	282	WP_003573774.1	type II toxin-antitoxin system YafQ family toxin	Toxin
BBD24_RS03435	684577..684975	+	399	WP_003586778.1	DUF1722 domain-containing protein	-
BBD24_RS03440	685220..686329	-	1110	WP_003607129.1	hypothetical protein	-
BBD24_RS03445	686331..687773	-	1443	WP_003586782.1	hypothetical protein	-
BBD24_RS03455	688046..688729	+	684	WP_002816607.1	IS6 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	608351..688729	80378
	-	flank	IS/Tn	-	-	677734..679149	1415

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (6-93)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-62)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11371.98 Da
• Isoelectric Point: 9.5081

>T64495 WP_003573774.1 NZ_CP016355:684096-684377 [Lacticaseibacillus paracasei subsp. paracasei]
MNKFVYKPAFERQFKKHYKTILKGGRYKKEDFEAVYHKLLRDEPLDPHYNDHALVNRKPERDLHIKPDWLLIYKYDGEFV
RFIDTGSHSDLFN

Nucleotide

• Length: 282 bp

>T64495 NZ_CP016355:684096-684377 [Lacticaseibacillus paracasei subsp. paracasei]
ATGAATAAATTTGTTTATAAACCAGCCTTTGAACGTCAATTTAAAAAACATTACAAGACGATTTTAAAAGGGGGGCGCTA
TAAAAAAGAAGATTTTGAAGCTGTTTATCATAAACTATTACGAGATGAACCGTTAGATCCGCATTATAATGATCACGCTT
TGGTTAATCGTAAACCAGAGCGTGATTTACATATCAAGCCGGATTGGTTATTAATTTACAAATATGATGGTGAGTTTGTG
CGCTTTATCGACACGGGTTCGCATTCAGATCTTTTTAACTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9217.44 Da
• Isoelectric Point: 5.1548

>AT64495 WP_016363783.1 NZ_CP016355:683861-684103 [Lacticaseibacillus paracasei subsp. paracasei]
MTSQVHFRMSEKEKKDFEIVLSRVGLTPGEAFRIFAKKSIEAGGIPFEVSQPNTRLKKAIRSQDYVEFNDPEEGLDWLNE

Nucleotide

• Length: 243 bp

>AT64495 NZ_CP016355:683861-684103 [Lacticaseibacillus paracasei subsp. paracasei]
ATGACTAGCCAAGTACATTTCAGAATGAGCGAAAAAGAAAAAAAGGACTTCGAGATTGTCCTAAGTCGTGTGGGGTTAAC
TCCCGGGGAAGCTTTTCGTATCTTTGCCAAGAAATCCATTGAAGCCGGAGGAATTCCCTTTGAGGTCTCTCAGCCAAACA
CACGTTTAAAAAAGGCTATTAGGAGTCAAGATTACGTTGAATTTAACGATCCTGAGGAAGGACTGGATTGGTTAAATGAA
TAA

Structures

Toxin

• AlphaFold DB: A0A243Q0H7