Detailed information of TA system TA064488

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3999241..3999838
• Replicon: chromosome
• Accession: NZ_CP016344
• Organism: Klebsiella variicola strain E57-7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B7GEF2
• Locus tag: BBB54_RS19855
• Protein ID: WP_012542526.1
• Coordinates: 3999521..3999838 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BBB54_RS19850
• Protein ID: WP_012542525.1
• Coordinates: 3999241..3999528 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBB54_RS19815	3995151..3995399	+	249	WP_008805539.1	DUF1158 domain-containing protein	-
BBB54_RS19820	3995416..3995757	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
BBB54_RS19825	3995788..3996903	-	1116	WP_162493283.1	MBL fold metallo-hydrolase	-
BBB54_RS19830	3997083..3997664	+	582	WP_012968754.1	TetR/AcrR family transcriptional regulator	-
BBB54_RS19835	3997664..3998032	+	369	WP_008805536.1	MmcQ/YjbR family DNA-binding protein	-
BBB54_RS19840	3998152..3998805	+	654	WP_012968755.1	oxygen-insensitive NAD(P)H nitroreductase	-
BBB54_RS19850	3999241..3999528	-	288	WP_012542525.1	helix-turn-helix transcriptional regulator	Antitoxin
BBB54_RS19855	3999521..3999838	-	318	WP_012542526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BBB54_RS19860	4000023..4001066	-	1044	WP_016160437.1	DUF2157 domain-containing protein	-
BBB54_RS19870	4001596..4002462	-	867	WP_008805530.1	helix-turn-helix transcriptional regulator	-
BBB54_RS19875	4002571..4003998	+	1428	WP_023297179.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T64488 WP_012542526.1 NZ_CP016344:c3999838-3999521 [Klebsiella variicola]
MFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T64488 NZ_CP016344:c3999838-3999521 [Klebsiella variicola]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10815.51 Da
• Isoelectric Point: 10.2212

>AT64488 WP_012542525.1 NZ_CP016344:c3999528-3999241 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Nucleotide

• Length: 288 bp

>AT64488 NZ_CP016344:c3999528-3999241 [Klebsiella variicola]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: A0A0B7GEF2