Detailed information of TA system TA064437

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 383936..384551
• Replicon: chromosome
• Accession: NZ_CP016325
• Organism: Vibrio cholerae 2740-80

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: BBB50_RS15770
• Protein ID: WP_001162670.1
• Coordinates: 383936..384223 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BBB50_RS15775
• Protein ID: WP_001232701.1
• Coordinates: 384234..384551 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBB50_RS20265	379221..379331	+	111	Protein_366	DUF3265 domain-containing protein	-
BBB50_RS20490	379572..379667	+	96	WP_001907607.1	DUF645 family protein	-
BBB50_RS15740	379861..380178	-	318	WP_001180243.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BBB50_RS15745	380197..380439	-	243	WP_000557292.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
BBB50_RS15750	380681..381190	+	510	WP_000405987.1	GNAT family N-acetyltransferase	-
BBB50_RS15755	381247..381900	+	654	WP_000226874.1	hypothetical protein	-
BBB50_RS15760	382210..382428	+	219	WP_001917086.1	DUF3709 domain-containing protein	-
BBB50_RS19775	382831..383064	+	234	WP_032482776.1	DUF3709 domain-containing protein	-
BBB50_RS15765	383489..383669	+	181	Protein_374	DUF645 family protein	-
BBB50_RS15770	383936..384223	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BBB50_RS15775	384234..384551	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
BBB50_RS20495	384548..384658	+	111	WP_134814058.1	DUF3265 domain-containing protein	-
BBB50_RS19790	384835..384960	+	126	WP_001944767.1	DUF645 family protein	-
BBB50_RS20275	384976..385014	+	39	WP_106019119.1	hypothetical protein	-
BBB50_RS15780	385238..385978	+	741	WP_000469836.1	PhzF family phenazine biosynthesis protein	-
BBB50_RS15785	386183..387013	+	831	WP_000056616.1	abortive infection family protein	-
BBB50_RS15790	387070..387552	+	483	WP_001895003.1	hypothetical protein	-
BBB50_RS15800	387999..388874	+	876	WP_000259044.1	hypothetical protein	-
BBB50_RS15805	389004..389459	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	304243..451810	147567
	-	inside	Integron	-	-	309323..451434	142111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T64437 WP_001162670.1 NZ_CP016325:383936-384223 [Vibrio cholerae 2740-80]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T64437 NZ_CP016325:383936-384223 [Vibrio cholerae 2740-80]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT64437 WP_001232701.1 NZ_CP016325:384234-384551 [Vibrio cholerae 2740-80]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT64437 NZ_CP016325:384234-384551 [Vibrio cholerae 2740-80]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTCAGTAATTTAATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCCGCGGCATTAGGTAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36