Detailed information of TA system TA064430

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 312696..313311
• Replicon: chromosome
• Accession: NZ_CP016325
• Organism: Vibrio cholerae 2740-80

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: BBB50_RS15290
• Protein ID: WP_001162670.1
• Coordinates: 312696..312983 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BBB50_RS15295
• Protein ID: WP_001232701.1
• Coordinates: 312994..313311 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBB50_RS15255	307997..308548	+	552	WP_001894072.1	translation initiation factor IF-3	-
BBB50_RS15260	308652..308846	+	195	WP_001885060.1	50S ribosomal protein L35	-
BBB50_RS15265	308888..309241	+	354	WP_001138366.1	50S ribosomal protein L20	-
BBB50_RS15270	309323..310285	-	963	WP_000841995.1	integron integrase IntIA	-
BBB50_RS19505	311075..311332	+	258	WP_080379067.1	hypothetical protein	-
BBB50_RS15275	311515..311928	+	414	WP_000049417.1	VOC family protein	-
BBB50_RS15285	312179..312415	+	237	WP_000868090.1	DUF645 family protein	-
BBB50_RS15290	312696..312983	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BBB50_RS15295	312994..313311	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
BBB50_RS20455	313308..313418	+	111	WP_172837665.1	DUF3265 domain-containing protein	-
BBB50_RS20460	313651..313746	+	96	WP_001907607.1	DUF645 family protein	-
BBB50_RS15300	314141..314725	+	585	WP_197668439.1	ATP-binding protein	-
BBB50_RS15305	315021..315200	+	180	WP_016711064.1	DUF645 family protein	-
BBB50_RS15310	315442..315672	-	231	WP_000838533.1	PAS factor family protein	-
BBB50_RS15315	315896..316753	+	858	WP_001894914.1	DUF2806 domain-containing protein	-
BBB50_RS15320	316900..317799	+	900	WP_001887493.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	304243..451810	147567
	-	inside	Integron	-	-	309323..451434	142111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T64430 WP_001162670.1 NZ_CP016325:312696-312983 [Vibrio cholerae 2740-80]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T64430 NZ_CP016325:312696-312983 [Vibrio cholerae 2740-80]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT64430 WP_001232701.1 NZ_CP016325:312994-313311 [Vibrio cholerae 2740-80]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT64430 NZ_CP016325:312994-313311 [Vibrio cholerae 2740-80]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTGAGTAATTTGATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCAGCGGCATTAGGCAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36