Detailed information of TA system TA064377

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3707570..3708162
• Replicon: chromosome
• Accession: NZ_CP016294
• Organism: Stenotrophomonas rhizophila strain QL-P4

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A1B2N862
• Locus tag: BAY15_RS16495
• Protein ID: WP_068854073.1
• Coordinates: 3707884..3708162 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BAY15_RS16490
• Protein ID: WP_083214286.1
• Coordinates: 3707570..3707872 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BAY15_RS16470	3702993..3704180	+	1188	WP_208856111.1	pyridoxal phosphate-dependent aminotransferase	-
BAY15_RS16475	3704318..3706411	-	2094	WP_068854069.1	prolyl oligopeptidase family protein	-
BAY15_RS16480	3706446..3706802	-	357	WP_068854070.1	hypothetical protein	-
BAY15_RS16485	3706967..3707569	+	603	WP_068854071.1	lipocalin family protein	-
BAY15_RS16490	3707570..3707872	-	303	WP_083214286.1	HigA family addiction module antidote protein	Antitoxin
BAY15_RS16495	3707884..3708162	-	279	WP_068854073.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BAY15_RS16500	3708295..3708984	-	690	WP_068854074.1	ABC transporter permease	-
BAY15_RS16505	3708981..3709988	-	1008	WP_068854815.1	methionine ABC transporter ATP-binding protein	-
BAY15_RS16510	3710121..3711017	-	897	WP_068854075.1	DMT family transporter	-
BAY15_RS16515	3711097..3711579	+	483	WP_068854076.1	YajQ family cyclic di-GMP-binding protein	-
BAY15_RS16520	3711586..3712179	+	594	WP_068854077.1	DUF1415 domain-containing protein	-
BAY15_RS16525	3712170..3712973	+	804	WP_068854078.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (24-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (8-84)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10759.44 Da
• Isoelectric Point: 10.3985

>T64377 WP_068854073.1 NZ_CP016294:c3708162-3707884 [Stenotrophomonas rhizophila]
MIQSFRCRHTLALFEGRCPRRFRSIQAVAERKLQMLDAAQTLQFLRSPPGNRLEALAGERRGQFSIRINVQWRVCFTWTD
AGPCHVEIVDYH

Nucleotide

• Length: 279 bp

>T64377 NZ_CP016294:c3708162-3707884 [Stenotrophomonas rhizophila]
ATGATCCAGTCCTTCCGCTGCCGCCACACGCTTGCCCTGTTCGAAGGCCGATGCCCACGGCGGTTCCGCAGCATCCAGGC
GGTTGCCGAGCGCAAGCTGCAGATGCTGGACGCGGCGCAGACCCTGCAGTTCCTGCGCAGTCCGCCCGGCAACCGTCTTG
AAGCGCTGGCGGGCGAACGACGAGGGCAGTTCAGCATCCGCATCAATGTCCAATGGCGCGTGTGTTTCACCTGGACGGAT
GCAGGTCCGTGCCATGTCGAAATCGTTGATTACCACTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11069.73 Da
• Isoelectric Point: 8.7939

>AT64377 WP_083214286.1 NZ_CP016294:c3707872-3707570 [Stenotrophomonas rhizophila]
MSRAANKLRAIHPGEILREEFMAPLELSINALARALDVPATRLHAIVHETRGVTADTAARLARHFGGDAASWLALQAEYD
LKTLSTWQDIERRVMPRVAA

Nucleotide

• Length: 303 bp

>AT64377 NZ_CP016294:c3707872-3707570 [Stenotrophomonas rhizophila]
ATGAGCCGTGCCGCCAACAAACTGAGGGCCATCCACCCGGGCGAAATCCTGCGGGAGGAATTCATGGCGCCGCTCGAGCT
GAGCATCAATGCGCTCGCGCGTGCCCTGGACGTGCCGGCCACCCGGCTGCATGCCATCGTGCATGAAACCAGGGGCGTCA
CCGCGGATACGGCGGCCCGGCTGGCGCGCCATTTCGGCGGTGACGCCGCCTCGTGGCTCGCACTGCAGGCCGAATACGAC
CTCAAGACCCTGTCTACGTGGCAGGACATCGAACGCAGGGTCATGCCGCGCGTTGCGGCCTGA

Structures

Toxin

• AlphaFold DB: A0A1B2N862