Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 496415..497029 Replicon chromosome
Accession NZ_CP016294
Organism Stenotrophomonas rhizophila strain QL-P4

Toxin (Protein)


Gene name higB Uniprot ID A0A1B2N011
Locus tag BAY15_RS02040 Protein ID WP_068848526.1
Coordinates 496715..497029 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1B2N027
Locus tag BAY15_RS02035 Protein ID WP_068848524.1
Coordinates 496415..496711 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BAY15_RS02010 491781..492671 + 891 WP_068848514.1 3-hydroxyisobutyrate dehydrogenase -
BAY15_RS02015 492835..493260 - 426 WP_068848516.1 organic hydroperoxide resistance protein -
BAY15_RS02020 493372..494202 + 831 WP_068848518.1 AraC family transcriptional regulator -
BAY15_RS19075 494195..494653 - 459 WP_157771668.1 hypothetical protein -
BAY15_RS02025 494840..495796 - 957 WP_068848520.1 cation transporter -
BAY15_RS02030 495906..496382 + 477 WP_068848522.1 hypothetical protein -
BAY15_RS02035 496415..496711 - 297 WP_068848524.1 helix-turn-helix domain-containing protein Antitoxin
BAY15_RS02040 496715..497029 - 315 WP_068848526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BAY15_RS18995 497333..497440 + 108 Protein_406 helix-turn-helix transcriptional regulator -
BAY15_RS02045 497430..497828 - 399 WP_068854502.1 hypothetical protein -
BAY15_RS19080 497885..498082 + 198 WP_157771669.1 hypothetical protein -
BAY15_RS02050 498077..498316 - 240 WP_068848528.1 hypothetical protein -
BAY15_RS02055 498582..499367 - 786 WP_068848530.1 hypothetical protein -
BAY15_RS02060 499360..499590 - 231 WP_068848532.1 hypothetical protein -
BAY15_RS19320 499587..499763 - 177 WP_167693286.1 hypothetical protein -
BAY15_RS19425 499756..500028 - 273 WP_068848534.1 AAA family ATPase -
BAY15_RS02070 500021..500446 - 426 WP_068848536.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(43-99)

Antitoxin

Cro/C1-type helix-turn-helix domain

(36-73)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11576.60 Da        Isoelectric Point: 10.1934

>T64375 WP_068848526.1 NZ_CP016294:c497029-496715 [Stenotrophomonas rhizophila]
MLFIETPTFTRAIKGLMPDDDYARFQLELAASPLVGDVIEGTGGLRKVRLGIPGRGKRGGARVIYYYIAPSSRIILLLVY
PKNVQDALTAEQKIALRSIIENWK

Download         Length: 315 bp

>T64375 NZ_CP016294:c497029-496715 [Stenotrophomonas rhizophila]
ATGCTCTTCATTGAAACGCCCACCTTTACCCGCGCGATCAAAGGACTGATGCCTGACGACGACTACGCCAGGTTCCAGCT
TGAGTTGGCCGCGTCGCCGCTGGTGGGAGATGTAATTGAGGGAACCGGTGGGCTGCGCAAGGTTCGCTTGGGAATCCCAG
GACGTGGGAAGCGTGGCGGTGCGAGGGTCATCTACTACTACATCGCCCCTTCGTCCCGGATCATCCTGCTTCTGGTGTAC
CCAAAGAATGTTCAGGACGCGCTGACCGCAGAACAGAAGATCGCACTCCGATCCATCATTGAGAACTGGAAGTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 10936.58 Da        Isoelectric Point: 10.2880

>AT64375 WP_068848524.1 NZ_CP016294:c496711-496415 [Stenotrophomonas rhizophila]
MDNTLFAELMGSVAEADEILKGNQVPSRELHVSPVQVREIRRATGLSQQKFARIIHVDVGTLRNWEQGRRDPTGPARALL
HVIMKNPKETLRVLAEAG

Download         Length: 297 bp

>AT64375 NZ_CP016294:c496711-496415 [Stenotrophomonas rhizophila]
ATGGACAACACCCTTTTTGCCGAACTGATGGGAAGCGTGGCTGAGGCCGATGAGATCCTGAAGGGTAATCAGGTACCGTC
GCGTGAGCTCCATGTCAGCCCCGTGCAAGTGCGCGAGATTCGCCGAGCGACGGGGCTGTCGCAGCAGAAATTCGCGCGCA
TCATCCACGTGGACGTTGGCACGCTGCGAAACTGGGAACAGGGGCGGCGGGACCCCACGGGTCCGGCACGTGCCCTGCTC
CACGTCATCATGAAGAATCCCAAGGAGACGCTTCGGGTGCTGGCGGAGGCGGGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B2N011

Antitoxin

Source ID Structure
AlphaFold DB A0A1B2N027

References