Detailed information of TA system TA064375

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 496415..497029
• Replicon: chromosome
• Accession: NZ_CP016294
• Organism: Stenotrophomonas rhizophila strain QL-P4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1B2N011
• Locus tag: BAY15_RS02040
• Protein ID: WP_068848526.1
• Coordinates: 496715..497029 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1B2N027
• Locus tag: BAY15_RS02035
• Protein ID: WP_068848524.1
• Coordinates: 496415..496711 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BAY15_RS02010	491781..492671	+	891	WP_068848514.1	3-hydroxyisobutyrate dehydrogenase	-
BAY15_RS02015	492835..493260	-	426	WP_068848516.1	organic hydroperoxide resistance protein	-
BAY15_RS02020	493372..494202	+	831	WP_068848518.1	AraC family transcriptional regulator	-
BAY15_RS19075	494195..494653	-	459	WP_157771668.1	hypothetical protein	-
BAY15_RS02025	494840..495796	-	957	WP_068848520.1	cation transporter	-
BAY15_RS02030	495906..496382	+	477	WP_068848522.1	hypothetical protein	-
BAY15_RS02035	496415..496711	-	297	WP_068848524.1	helix-turn-helix domain-containing protein	Antitoxin
BAY15_RS02040	496715..497029	-	315	WP_068848526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BAY15_RS18995	497333..497440	+	108	Protein_406	helix-turn-helix transcriptional regulator	-
BAY15_RS02045	497430..497828	-	399	WP_068854502.1	hypothetical protein	-
BAY15_RS19080	497885..498082	+	198	WP_157771669.1	hypothetical protein	-
BAY15_RS02050	498077..498316	-	240	WP_068848528.1	hypothetical protein	-
BAY15_RS02055	498582..499367	-	786	WP_068848530.1	hypothetical protein	-
BAY15_RS02060	499360..499590	-	231	WP_068848532.1	hypothetical protein	-
BAY15_RS19320	499587..499763	-	177	WP_167693286.1	hypothetical protein	-
BAY15_RS19425	499756..500028	-	273	WP_068848534.1	AAA family ATPase	-
BAY15_RS02070	500021..500446	-	426	WP_068848536.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-99)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-73)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11576.60 Da
• Isoelectric Point: 10.1934

>T64375 WP_068848526.1 NZ_CP016294:c497029-496715 [Stenotrophomonas rhizophila]
MLFIETPTFTRAIKGLMPDDDYARFQLELAASPLVGDVIEGTGGLRKVRLGIPGRGKRGGARVIYYYIAPSSRIILLLVY
PKNVQDALTAEQKIALRSIIENWK

Nucleotide

• Length: 315 bp

>T64375 NZ_CP016294:c497029-496715 [Stenotrophomonas rhizophila]
ATGCTCTTCATTGAAACGCCCACCTTTACCCGCGCGATCAAAGGACTGATGCCTGACGACGACTACGCCAGGTTCCAGCT
TGAGTTGGCCGCGTCGCCGCTGGTGGGAGATGTAATTGAGGGAACCGGTGGGCTGCGCAAGGTTCGCTTGGGAATCCCAG
GACGTGGGAAGCGTGGCGGTGCGAGGGTCATCTACTACTACATCGCCCCTTCGTCCCGGATCATCCTGCTTCTGGTGTAC
CCAAAGAATGTTCAGGACGCGCTGACCGCAGAACAGAAGATCGCACTCCGATCCATCATTGAGAACTGGAAGTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10936.58 Da
• Isoelectric Point: 10.2880

>AT64375 WP_068848524.1 NZ_CP016294:c496711-496415 [Stenotrophomonas rhizophila]
MDNTLFAELMGSVAEADEILKGNQVPSRELHVSPVQVREIRRATGLSQQKFARIIHVDVGTLRNWEQGRRDPTGPARALL
HVIMKNPKETLRVLAEAG

Nucleotide

• Length: 297 bp

>AT64375 NZ_CP016294:c496711-496415 [Stenotrophomonas rhizophila]
ATGGACAACACCCTTTTTGCCGAACTGATGGGAAGCGTGGCTGAGGCCGATGAGATCCTGAAGGGTAATCAGGTACCGTC
GCGTGAGCTCCATGTCAGCCCCGTGCAAGTGCGCGAGATTCGCCGAGCGACGGGGCTGTCGCAGCAGAAATTCGCGCGCA
TCATCCACGTGGACGTTGGCACGCTGCGAAACTGGGAACAGGGGCGGCGGGACCCCACGGGTCCGGCACGTGCCCTGCTC
CACGTCATCATGAAGAATCCCAAGGAGACGCTTCGGGTGCTGGCGGAGGCGGGTTGA

Structures

Toxin

• AlphaFold DB: A0A1B2N011

Antitoxin

• AlphaFold DB: A0A1B2N027