Detailed information of TA system TA064361

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1183..1823
• Replicon: plasmid pPCP1
• Accession: NZ_CP016276
• Organism: Yersinia pestis strain Cadman

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: BAY22_RS22250
• Protein ID: WP_002225538.1
• Coordinates: 1183..1524 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: Q9S1D9
• Locus tag: BAY22_RS22255
• Protein ID: WP_002213446.1
• Coordinates: 1524..1823 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BAY22_RS22250	1183..1524	+	342	WP_002225538.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BAY22_RS22255	1524..1823	+	300	WP_002213446.1	XRE family transcriptional regulator	Antitoxin
BAY22_RS22260	2010..2948	-	939	WP_002218234.1	omptin family plasminogen activator Pla	-
BAY22_RS22265	3191..3496	-	306	WP_002221218.1	hypothetical protein	-
BAY22_RS22270	3725..4798	+	1074	WP_002218509.1	pesticin	-
BAY22_RS22275	4833..5258	-	426	WP_002218512.1	pesticin immunity protein	-
BAY22_RS22280	6494..6688	-	195	WP_002213433.1	Rop family plasmid primer RNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	pla	1..9612	9612

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (10-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13288.22 Da
• Isoelectric Point: 8.8337

>T64361 WP_002225538.1 NZ_CP016276:1183-1524 [Yersinia pestis]
MVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAIVL
CAGDKSNDKRFYEKLVRIAEDEFTAHLNTLESK

Nucleotide

• Length: 342 bp

>T64361 NZ_CP016276:1183-1524 [Yersinia pestis]
ATGGTACTTTTTAGCCAGCGATTTGACGACTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGCTGATTT
GAAAAAGCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTGAAAGGTTCTCGGTACAAAAATATGA
AAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATAAGAGCCTTTTATGCGTTCGATCCGATTCGTCGGGCTATCGTTCTT
TGTGCAGGAGATAAAAGTAATGATAAGCGGTTTTATGAAAAACTGGTGCGTATAGCTGAGGATGAATTTACAGCACATCT
GAACACACTGGAGAGCAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11127.94 Da
• Isoelectric Point: 5.8900

>AT64361 WP_002213446.1 NZ_CP016276:1524-1823 [Yersinia pestis]
MRTLDEVIASRSPESQTRIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGRRVAFHV

Nucleotide

• Length: 300 bp

>AT64361 NZ_CP016276:1524-1823 [Yersinia pestis]
ATGAGAACATTAGATGAGGTGATTGCCAGTCGTTCACCTGAAAGCCAGACACGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAACAAGTTGCTGAGGCGATGGGTATAAGCC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACGTTAAAGCGTTACGTTGAAGCAATGGGA
GGCAAATTAAGCTTGGATGTTGAGCTTCCTACAGGAAGGAGAGTAGCGTTCCATGTCTAA

Structures

Antitoxin

• AlphaFold DB: A0A080J016