Detailed information of TA system TA064335

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 2175948..2176543
• Replicon: chromosome
• Accession: NZ_CP016214
• Organism: Pseudomonas aeruginosa strain PA121617

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: A9P90_RS32130
• Protein ID: WP_003113526.1
• Coordinates: 2176265..2176543 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A9P90_RS10030
• Protein ID: WP_003099268.1
• Coordinates: 2175948..2176253 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9P90_RS10020	2171325..2171656	+	332	Protein_1985	hypothetical protein	-
A9P90_RS10025	2171923..2172939	+	1017	WP_032728035.1	IS30-like element IS1394 family transposase	-
A9P90_RS33140	2172945..2175500	+	2556	WP_172832407.1	S1 RNA-binding domain-containing protein	-
A9P90_RS10030	2175948..2176253	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
A9P90_RS32130	2176265..2176543	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A9P90_RS10035	2176872..2179100	+	2229	WP_003099265.1	TonB-dependent receptor	-
A9P90_RS10040	2179170..2179817	-	648	WP_003095021.1	carbonate dehydratase	-
A9P90_RS10045	2179879..2181117	-	1239	WP_019681675.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2171923..2172939	1016

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T64335 WP_003113526.1 NZ_CP016214:c2176543-2176265 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T64335 NZ_CP016214:c2176543-2176265 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT64335 WP_003099268.1 NZ_CP016214:c2176253-2175948 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT64335 NZ_CP016214:c2176253-2175948 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCCGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3