Detailed information of TA system TA064330

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 1865267..1865967
• Replicon: chromosome
• Accession: NZ_CP016212
• Organism: Pseudomonas putida JB

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: -
• Locus tag: Q5O_RS08240
• Protein ID: WP_041166873.1
• Coordinates: 1865267..1865563 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: I7AUJ7
• Locus tag: Q5O_RS08245
• Protein ID: WP_003254235.1
• Coordinates: 1865566..1865967 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Q5O_RS08220	1861259..1862437	-	1179	WP_014590258.1	efflux RND transporter periplasmic adaptor subunit	-
Q5O_RS08225	1862650..1863126	+	477	WP_014590259.1	sigma-70 family RNA polymerase sigma factor	-
Q5O_RS08230	1863295..1863774	+	480	WP_014590260.1	hypothetical protein	-
Q5O_RS08235	1863847..1865172	+	1326	WP_014590261.1	MFS transporter	-
Q5O_RS08240	1865267..1865563	+	297	WP_041166873.1	type II toxin-antitoxin system MqsR family toxin	Toxin
Q5O_RS08245	1865566..1865967	+	402	WP_003254235.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
Q5O_RS08250	1866043..1867725	-	1683	WP_080557776.1	electron transfer flavoprotein-ubiquinone oxidoreductase	-
Q5O_RS08255	1868261..1869010	+	750	WP_014590265.1	electron transfer flavoprotein subunit beta/FixA family protein	-
Q5O_RS08260	1869011..1869940	+	930	WP_003254230.1	FAD-binding protein	-
Q5O_RS08265	1870016..1870837	+	822	WP_014590266.1	transporter substrate-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (6-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (74-109)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11105.16 Da
• Isoelectric Point: 10.0317

>T64330 WP_041166873.1 NZ_CP016212:1865267-1865563 [Pseudomonas putida JB]
MEKRMPHCPLERVKALAAARRIRPTGAALKGAKALGMDFPGMLEVITSLKRTDFYKSMTSHIDHQVWQDVYRPLTAIGYV
YLKLSVVDDVLIVSFKEL

Nucleotide

• Length: 297 bp

>T64330 NZ_CP016212:1865267-1865563 [Pseudomonas putida JB]
ATGGAAAAGAGAATGCCTCATTGCCCGCTGGAGCGGGTAAAAGCCCTGGCTGCGGCAAGGCGAATCCGTCCTACGGGTGC
TGCATTGAAAGGCGCCAAGGCGCTGGGAATGGATTTTCCGGGCATGCTTGAGGTGATCACCAGCTTAAAGCGCACTGATT
TCTACAAGAGCATGACCAGCCATATTGACCACCAGGTTTGGCAGGATGTGTATCGCCCGCTTACAGCAATTGGGTACGTT
TATTTGAAATTGTCCGTGGTAGACGACGTGCTCATCGTGTCTTTCAAGGAGTTGTAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14684.76 Da
• Isoelectric Point: 4.9531

>AT64330 WP_003254235.1 NZ_CP016212:1865566-1865967 [Pseudomonas putida JB]
MRCPICGGSELAPDIQGMPYSYKGEMTVIPEVSGDYCSACGECVLSHDEAMRVSHLMTAFERQVNANVVDPSFIASIRRK
FDLDQREAGEIFGGGVNAFSRYENGKTTPPVALVKLLKLLDRHPELFEEVRTA

Nucleotide

• Length: 402 bp

>AT64330 NZ_CP016212:1865566-1865967 [Pseudomonas putida JB]
ATGAGATGTCCAATATGCGGCGGCTCGGAGCTTGCACCTGACATTCAGGGCATGCCCTATAGCTACAAAGGCGAGATGAC
AGTGATCCCGGAGGTAAGTGGCGATTACTGCTCTGCATGTGGTGAATGCGTACTGAGTCATGATGAAGCCATGCGCGTCA
GTCACTTGATGACGGCATTCGAGCGCCAGGTCAACGCAAATGTTGTGGATCCTTCCTTTATTGCCTCCATACGCAGAAAG
TTCGACCTCGACCAGCGTGAAGCGGGGGAAATCTTCGGTGGTGGGGTCAATGCGTTCTCCCGTTATGAAAACGGCAAGAC
CACCCCGCCAGTGGCGTTGGTGAAACTGCTCAAGCTGCTGGATCGCCATCCAGAACTCTTTGAGGAAGTGCGTACTGCCT
GA

Structures

Antitoxin

• AlphaFold DB: I7AUJ7