Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3974987..3975681 Replicon chromosome
Accession NZ_CP016182
Organism Escherichia coli strain EC590

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag BAY41_RS20530 Protein ID WP_001263489.1
Coordinates 3974987..3975385 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag BAY41_RS20535 Protein ID WP_000554758.1
Coordinates 3975388..3975681 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3970575..3970655 - 81 NuclAT_12 - -
- 3970575..3970655 - 81 NuclAT_12 - -
- 3970575..3970655 - 81 NuclAT_12 - -
- 3970575..3970655 - 81 NuclAT_12 - -
BAY41_RS20500 3971251..3971709 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
BAY41_RS20505 3971970..3973427 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
BAY41_RS20510 3973484..3974005 - 522 Protein_3736 peptide chain release factor H -
BAY41_RS20515 3974001..3974207 - 207 Protein_3737 RtcB family protein -
BAY41_RS20525 3974525..3974977 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
BAY41_RS20530 3974987..3975385 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
BAY41_RS20535 3975388..3975681 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
BAY41_RS20540 3975733..3976788 - 1056 WP_001226164.1 DNA polymerase IV -
BAY41_RS20545 3976859..3977644 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
BAY41_RS20550 3977616..3979328 + 1713 Protein_3743 flagellar biosynthesis protein FlhA -
BAY41_RS20555 3979552..3980049 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T64324 WP_001263489.1 NZ_CP016182:c3975385-3974987 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T64324 NZ_CP016182:c3975385-3974987 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT64324 WP_000554758.1 NZ_CP016182:c3975681-3975388 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT64324 NZ_CP016182:c3975681-3975388 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References