Detailed information of TA system TA064302

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 1214489..1215122
• Replicon: chromosome
• Accession: NZ_CP016178
• Organism: Vibrio breoganii strain FF50

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A193KIJ2
• Locus tag: A6E01_RS18275
• Protein ID: WP_017028234.1
• Coordinates: 1214489..1214821 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A6E01_RS18280
• Protein ID: WP_017028235.1
• Coordinates: 1214808..1215122 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6E01_RS18255	1209899..1210321	+	423	WP_017028230.1	organic hydroperoxide resistance protein	-
A6E01_RS18260	1210393..1211322	+	930	WP_065210947.1	NADP-dependent oxidoreductase	-
A6E01_RS18265	1211560..1213020	-	1461	WP_017028232.1	N-acetylglucosamine-specific PTS transporter subunit IIBC	-
A6E01_RS18270	1213222..1214106	+	885	WP_017028233.1	LysR family transcriptional regulator	-
A6E01_RS18275	1214489..1214821	+	333	WP_017028234.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A6E01_RS18280	1214808..1215122	+	315	WP_017028235.1	DNA-binding transcriptional regulator	Antitoxin
A6E01_RS18285	1215284..1216327	-	1044	WP_065210948.1	DUF1254 domain-containing protein	-
A6E01_RS18290	1216591..1217835	-	1245	WP_017028237.1	NADH:flavin oxidoreductase/NADH oxidase family protein	-
A6E01_RS18295	1218055..1218285	-	231	WP_026012860.1	DUF3622 domain-containing protein	-
A6E01_RS18300	1218681..1219556	+	876	WP_017031637.1	5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-104)

Antitoxin

• Antitoxin MqsA/HigA-2 (18-101)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12948.80 Da
• Isoelectric Point: 10.1187

>T64302 WP_017028234.1 NZ_CP016178:1214489-1214821 [Vibrio breoganii]
MKSIFVESTIFEKYRNEYLSDDEFRLFQAELMSNPKQGDVIQGTGGLRKIRVASKGKGKRGGSRVIYYFLDERRRFYLLT
IYGKNEMSDLTADQKKQLKAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T64302 NZ_CP016178:1214489-1214821 [Vibrio breoganii]
ATGAAAAGTATATTTGTCGAATCAACCATATTTGAAAAGTATCGCAATGAATATCTAAGTGATGATGAGTTTAGGCTTTT
TCAAGCTGAGTTAATGTCTAACCCAAAACAAGGGGACGTGATTCAGGGTACAGGTGGTTTGCGAAAGATTCGAGTTGCAA
GTAAAGGTAAGGGCAAGCGTGGCGGGTCTCGGGTTATCTATTACTTCCTCGATGAAAGACGTCGTTTCTACTTACTGACA
ATTTATGGCAAGAATGAAATGTCCGATTTAACCGCAGACCAAAAGAAACAACTAAAGGCTTTTATGGAGGCGTGGCGTAA
TGAGCAATCGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11713.14 Da
• Isoelectric Point: 6.2155

>AT64302 WP_017028235.1 NZ_CP016178:1214808-1215122 [Vibrio breoganii]
MSNRDLFAELSSALVEAQEHSEGKLTLKTHHVNNISELDISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSAP
NGQAVTLLKLVQRHPETLSHIAEL

Nucleotide

• Length: 315 bp

>AT64302 NZ_CP016178:1214808-1215122 [Vibrio breoganii]
ATGAGCAATCGTGATCTATTTGCAGAGTTAAGTTCAGCTCTTGTTGAGGCTCAAGAGCATTCCGAGGGTAAACTAACCCT
TAAAACTCATCATGTTAACAATATCAGTGAGCTAGATATTTCACCTGATGAAATCGTGAGTATTCGTGAGCAGTTTAATA
TGTCGCGTGGTGTGTTTGCGCGTTTGCTGCATACATCATCTCGTACACTAGAAAACTGGGAACAAGGGCGTAGCGCACCA
AATGGACAAGCGGTAACCCTTCTAAAATTGGTTCAGCGTCATCCTGAAACTCTGTCTCATATCGCTGAACTATAA

Structures

Toxin

• AlphaFold DB: A0A193KIJ2