Detailed information of TA system TA064294

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 427895..428527
• Replicon: chromosome
• Accession: NZ_CP016176
• Organism: Xenorhabdus hominickii strain ANU1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2G0Q6D7
• Locus tag: A9255_RS01900
• Protein ID: WP_069315226.1
• Coordinates: 427895..428182 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A9255_RS01905
• Protein ID: WP_198148733.1
• Coordinates: 428228..428527 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9255_RS01880	424413..425555	+	1143	WP_069315222.1	FMN-dependent L-lactate dehydrogenase LldD	-
A9255_RS01885	425612..426118	-	507	WP_069315223.1	DUF1543 domain-containing protein	-
A9255_RS01890	426194..426982	-	789	WP_069315224.1	PhzF family phenazine biosynthesis protein	-
A9255_RS01895	427155..427601	+	447	WP_069315225.1	hypothetical protein	-
A9255_RS01900	427895..428182	+	288	WP_069315226.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A9255_RS01905	428228..428527	+	300	WP_198148733.1	HigA family addiction module antidote protein	Antitoxin
A9255_RS01910	428765..430300	-	1536	WP_069315228.1	peptide MFS transporter	-
A9255_RS01915	430879..431820	-	942	WP_069315229.1	LpxL/LpxP family Kdo(2)-lipid IV(A) lauroyl/palmitoleoyl acyltransferasee	-
A9255_RS01920	432480..432695	+	216	WP_071933821.1	type II toxin-antitoxin system YafO family toxin	-
A9255_RS01925	432916..433305	-	390	WP_069315230.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	417808..439306	21498
	-	flank	IS/Tn	-	-	423102..423455	353

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-68)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11385.98 Da
• Isoelectric Point: 7.1325

>T64294 WP_069315226.1 NZ_CP016176:427895-428182 [Xenorhabdus hominickii]
MAVEFKERWLERFYEDDLSHKCIPAALSNTLYRKLQMLDAATEENDLRAPPGNDFERLKGNLQGWCSIRVNRKYRLIFQW
MDKTAVDTYLDPHKY

Nucleotide

• Length: 288 bp

>T64294 NZ_CP016176:427895-428182 [Xenorhabdus hominickii]
ATGGCAGTTGAATTCAAGGAACGATGGTTGGAACGGTTTTATGAGGATGACCTTTCCCATAAATGTATTCCTGCGGCATT
AAGCAATACTTTGTACCGAAAATTGCAAATGTTGGATGCTGCGACGGAAGAGAATGATTTAAGGGCACCACCAGGTAACG
ATTTTGAACGTTTAAAAGGGAATTTACAGGGATGGTGCTCAATAAGGGTAAACAGGAAGTATCGCTTAATTTTCCAATGG
ATGGATAAAACAGCGGTTGATACTTATTTGGATCCTCATAAGTACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11173.99 Da
• Isoelectric Point: 7.4053

>AT64294 WP_198148733.1 NZ_CP016176:428228-428527 [Xenorhabdus hominickii]
MMRNTKRRPATVGEILISEFLEPLGIEINVLADAMGVHRNSLSRLIHDKGTLTAPMAIKLGATLGNTAEFWLNIQHAVEV
WDVRHQESEMNTKDIKRLI

Nucleotide

• Length: 300 bp

>AT64294 NZ_CP016176:428228-428527 [Xenorhabdus hominickii]
ATGATGAGAAATACTAAACGTCGCCCAGCAACTGTGGGTGAAATACTTATAAGTGAGTTTCTTGAACCTCTGGGGATAGA
AATCAACGTATTGGCCGATGCAATGGGTGTACATAGGAATTCGCTGAGTCGCTTGATCCATGATAAAGGAACCTTAACAG
CACCAATGGCAATTAAATTGGGGGCTACGTTAGGAAATACGGCTGAATTTTGGTTGAATATTCAACATGCTGTTGAGGTC
TGGGATGTTCGTCATCAAGAGAGCGAGATGAATACCAAGGACATTAAACGGTTAATTTAG

Structures

Toxin

• AlphaFold DB: A0A2G0Q6D7