Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1099259..1099856 Replicon chromosome
Accession NZ_CP016159
Organism Klebsiella pneumoniae strain TH1

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag BAU11_RS05365 Protein ID WP_004142563.1
Coordinates 1099539..1099856 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag BAU11_RS05360 Protein ID WP_004142561.1
Coordinates 1099259..1099546 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BAU11_RS05330 1095339..1095587 + 249 WP_002893037.1 DUF1158 domain-containing protein -
BAU11_RS05335 1095605..1095946 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
BAU11_RS05340 1095977..1097092 - 1116 WP_074188732.1 MBL fold metallo-hydrolase -
BAU11_RS05345 1097272..1097853 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
BAU11_RS05350 1097853..1098221 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
BAU11_RS05355 1098341..1098994 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
BAU11_RS05360 1099259..1099546 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
BAU11_RS05365 1099539..1099856 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BAU11_RS05370 1100041..1101084 - 1044 WP_020802450.1 DUF2157 domain-containing protein -
BAU11_RS05375 1101754..1102620 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
BAU11_RS05380 1102729..1104156 + 1428 WP_032411778.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T64245 WP_004142563.1 NZ_CP016159:c1099856-1099539 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T64245 NZ_CP016159:c1099856-1099539 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT64245 WP_004142561.1 NZ_CP016159:c1099546-1099259 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT64245 NZ_CP016159:c1099546-1099259 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTAGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References