Detailed information of TA system TA064213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3810999..3811612
• Replicon: chromosome
• Accession: NZ_CP016079
• Organism: Mesorhizobium loti NZP2037

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A807CB23
• Locus tag: A9174_RS18340
• Protein ID: WP_019859110.1
• Coordinates: 3811316..3811612 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A9174_RS18335
• Protein ID: WP_019859111.1
• Coordinates: 3810999..3811307 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9174_RS36390	3806905..3807021	-	117	Protein_3676	SDR family oxidoreductase	-
A9174_RS18320	3807117..3808295	-	1179	WP_019859115.1	lytic murein transglycosylase	-
A9174_RS18325	3808416..3809216	-	801	WP_027047027.1	glycoside hydrolase family 25 protein	-
A9174_RS18330	3809505..3810884	+	1380	WP_019859113.1	serine hydrolase	-
A9174_RS18335	3810999..3811307	-	309	WP_019859111.1	HigA family addiction module antidote protein	Antitoxin
A9174_RS18340	3811316..3811612	-	297	WP_019859110.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A9174_RS18345	3811731..3812414	-	684	WP_019859109.1	class I SAM-dependent methyltransferase	-
A9174_RS18350	3812411..3813709	-	1299	WP_019859108.1	DUF3419 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11680.15 Da
• Isoelectric Point: 6.8806

>T64213 WP_019859110.1 NZ_CP016079:c3811612-3811316 [Mesorhizobium loti NZP2037]
MIVGFRDGWLRAFFVDDTHSRNIPSDLESRLFRKLQMIDDAMVDRDLRVPPSNHFEKLRGNLEGFHSIRVNQQWRLIFRW
DGGRGEASDIYLDDHSYK

Nucleotide

• Length: 297 bp

>T64213 NZ_CP016079:c3811612-3811316 [Mesorhizobium loti NZP2037]
ATGATCGTCGGTTTTAGAGACGGATGGCTGCGGGCCTTCTTTGTAGACGACACCCACTCCCGCAACATTCCGTCCGATCT
CGAAAGCCGCTTGTTCCGCAAGCTCCAGATGATTGACGACGCCATGGTCGATCGGGATTTGCGCGTGCCGCCCAGCAACC
ATTTCGAGAAGTTGCGCGGCAATCTCGAGGGCTTCCATTCGATCCGCGTCAACCAGCAGTGGCGACTGATCTTCCGCTGG
GACGGCGGTCGCGGTGAAGCGTCAGACATTTATCTCGACGACCACAGTTATAAGTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11332.09 Da
• Isoelectric Point: 9.4051

>AT64213 WP_019859111.1 NZ_CP016079:c3811307-3810999 [Mesorhizobium loti NZP2037]
MLMTARKPATVGEILTEEFMQPLGLTQAALAEAMGVQRKHVNELCNDRRNVTAATALILARVFGNSPDFWLNVQRRSDLW
AVMNSPDERARVDRAKPLPTAA

Nucleotide

• Length: 309 bp

>AT64213 NZ_CP016079:c3811307-3810999 [Mesorhizobium loti NZP2037]
ATGTTGATGACCGCACGCAAGCCAGCAACGGTTGGCGAGATTCTCACCGAGGAGTTCATGCAGCCGCTCGGCTTGACGCA
GGCGGCATTGGCCGAGGCGATGGGCGTTCAGCGCAAGCATGTGAATGAATTGTGCAACGACCGTCGCAACGTGACGGCGG
CAACCGCGCTCATTCTGGCCCGGGTGTTTGGCAACAGCCCGGACTTCTGGCTGAATGTCCAACGCCGCAGCGATCTTTGG
GCGGTTATGAATTCGCCCGACGAGCGGGCACGTGTTGACCGCGCCAAGCCTTTACCGACAGCCGCATAG

Structures

Toxin

• AlphaFold DB: A0A807CB23