Detailed information of TA system TA064211

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2402235..2402828
• Replicon: chromosome
• Accession: NZ_CP016079
• Organism: Mesorhizobium loti NZP2037

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A807C4U3
• Locus tag: A9174_RS11680
• Protein ID: WP_019860610.1
• Coordinates: 2402547..2402828 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A9174_RS11675
• Protein ID: WP_019860611.1
• Coordinates: 2402235..2402534 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9174_RS38350	2397291..2397428	-	138	WP_019860621.1	hypothetical protein	-
A9174_RS11635	2397468..2397734	-	267	WP_019860620.1	hypothetical protein	-
A9174_RS11640	2397731..2398000	-	270	WP_019860618.1	hypothetical protein	-
A9174_RS11645	2398170..2398376	-	207	WP_010911078.1	cold-shock protein	-
A9174_RS11650	2398700..2398924	+	225	WP_019860617.1	hypothetical protein	-
A9174_RS11655	2399111..2400745	+	1635	WP_027047292.1	tetratricopeptide repeat-containing sulfotransferase family protein	-
A9174_RS11660	2400890..2401243	-	354	WP_019860615.1	DUF1428 domain-containing protein	-
A9174_RS11665	2401336..2401758	-	423	WP_019860613.1	glyoxalase/bleomycin resistance/extradiol dioxygenase family protein	-
A9174_RS11670	2401919..2402101	-	183	WP_032920719.1	hypothetical protein	-
A9174_RS11675	2402235..2402534	-	300	WP_019860611.1	HigA family addiction module antidote protein	Antitoxin
A9174_RS11680	2402547..2402828	-	282	WP_019860610.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A9174_RS11685	2402946..2404472	-	1527	WP_019860609.1	ATP-binding cassette domain-containing protein	-
A9174_RS11690	2404722..2405627	-	906	WP_019860608.1	DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10577.92 Da
• Isoelectric Point: 9.0992

>T64211 WP_019860610.1 NZ_CP016079:c2402828-2402547 [Mesorhizobium loti NZP2037]
MIRSFKDKTSEAVSNGKSPKGFPSDLVRSAQRKLFMIDNATELSDLKTPPGNRLEPLKGNRAGQHSIRINDQFRICFRWV
DGQAEDVEITDYH

Nucleotide

• Length: 282 bp

>T64211 NZ_CP016079:c2402828-2402547 [Mesorhizobium loti NZP2037]
ATGATACGCTCGTTCAAGGACAAGACCAGCGAGGCCGTTTCGAATGGCAAATCTCCAAAGGGCTTCCCGTCGGACCTTGT
TCGATCGGCCCAGCGGAAACTGTTCATGATCGACAACGCGACAGAGTTGTCGGACCTGAAGACTCCCCCAGGCAACAGGC
TCGAGCCCTTGAAGGGCAACCGTGCCGGTCAGCATTCGATCCGCATCAACGACCAGTTTCGCATCTGCTTTCGCTGGGTG
GACGGGCAGGCTGAGGATGTCGAAATAACTGACTACCATTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11131.91 Da
• Isoelectric Point: 6.4689

>AT64211 WP_019860611.1 NZ_CP016079:c2402534-2402235 [Mesorhizobium loti NZP2037]
MLNINPPIHPGEILREEYLVPLGLKPYTLAKKLHVPRTRIERLASEATPVTPDTALRLAKFFGTTPQFWMNMQASYDLAV
EGEAKKADLETIERLDTAA

Nucleotide

• Length: 300 bp

>AT64211 NZ_CP016079:c2402534-2402235 [Mesorhizobium loti NZP2037]
ATGCTGAACATCAACCCTCCCATTCATCCCGGAGAAATCCTTCGAGAGGAATACCTCGTCCCGCTTGGCCTGAAGCCTTA
CACGCTAGCCAAAAAGCTGCATGTGCCGCGCACGCGCATCGAGCGCCTGGCAAGCGAGGCGACACCGGTAACGCCCGACA
CCGCGCTACGCCTTGCCAAGTTTTTCGGAACCACGCCGCAATTCTGGATGAACATGCAGGCGAGCTACGACCTTGCCGTA
GAGGGAGAGGCCAAGAAAGCCGACCTTGAAACGATCGAGCGCTTGGACACAGCGGCCTGA

Structures

Toxin

• AlphaFold DB: A0A807C4U3