Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3607040..3607734 Replicon chromosome
Accession NZ_CP016018
Organism Escherichia coli strain ER1821R

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag A9X67_RS17915 Protein ID WP_001263489.1
Coordinates 3607040..3607438 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag A9X67_RS17920 Protein ID WP_000554758.1
Coordinates 3607441..3607734 (-) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3602628..3602708 - 81 NuclAT_12 - -
- 3602628..3602708 - 81 NuclAT_12 - -
- 3602628..3602708 - 81 NuclAT_12 - -
- 3602628..3602708 - 81 NuclAT_12 - -
A9X67_RS17890 3603304..3603762 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
A9X67_RS17895 3604023..3605480 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
A9X67_RS17900 3605537..3606058 - 522 Protein_3422 peptide chain release factor H -
A9X67_RS17905 3606054..3606260 - 207 Protein_3423 RtcB family protein -
A9X67_RS17910 3606578..3607030 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
A9X67_RS17915 3607040..3607438 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
A9X67_RS17920 3607441..3607734 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
A9X67_RS17925 3607786..3608841 - 1056 WP_001226164.1 DNA polymerase IV -
A9X67_RS17930 3608912..3609697 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
A9X67_RS17935 3609669..3611381 + 1713 Protein_3429 flagellar biosynthesis protein FlhA -
A9X67_RS17940 3611605..3612102 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-130)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T64144 WP_001263489.1 NZ_CP016018:c3607438-3607040 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T64144 NZ_CP016018:c3607438-3607040 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT64144 WP_000554758.1 NZ_CP016018:c3607734-3607441 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT64144 NZ_CP016018:c3607734-3607441 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A090J8B1


Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References