Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1789717..1790219 Replicon chromosome
Accession NZ_CP016018
Organism Escherichia coli strain ER1821R

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag A9X67_RS08695 Protein ID WP_000767829.1
Coordinates 1789965..1790219 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag A9X67_RS08690 Protein ID WP_001259255.1
Coordinates 1789717..1789968 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9X67_RS08670 1784895..1785962 - 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
A9X67_RS08675 1785962..1787032 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
A9X67_RS08680 1787029..1788333 - 1305 WP_000009594.1 histidinol dehydrogenase -
A9X67_RS08685 1788339..1789238 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
A9X67_RS23335 1789384..1789434 - 51 WP_001364200.1 his operon leader peptide -
A9X67_RS08690 1789717..1789968 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
A9X67_RS08695 1789965..1790219 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
A9X67_RS08700 1790302..1791126 + 825 WP_000754737.1 SDR family oxidoreductase -
A9X67_RS08705 1791172..1792101 + 930 WP_000803351.1 LysR family transcriptional regulator -
A9X67_RS25240 1792316..1792378 + 63 WP_010723108.1 membrane protein YoeI -
A9X67_RS08710 1792368..1793726 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
A9X67_RS08715 1793905..1794963 + 1059 WP_000492339.1 transport protein YeeE -
A9X67_RS08720 1794977..1795204 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T64129 WP_000767829.1 NZ_CP016018:1789965-1790219 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T64129 NZ_CP016018:1789965-1790219 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT64129 WP_001259255.1 NZ_CP016018:1789717-1789968 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT64129 NZ_CP016018:1789717-1789968 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References