Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4985461..4986014 Replicon chromosome
Accession NZ_CP016007
Organism Escherichia coli strain NGF1

Toxin (Protein)


Gene name higB Uniprot ID A0A0G9FUJ7
Locus tag A9Z04_RS24275 Protein ID WP_000605680.1
Coordinates 4985745..4986014 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag A9Z04_RS24270 Protein ID WP_000781370.1
Coordinates 4985461..4985745 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9Z04_RS24255 4980642..4983689 + 3048 WP_077634977.1 formate dehydrogenase-N subunit alpha -
A9Z04_RS24260 4983702..4984586 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
A9Z04_RS24265 4984579..4985232 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
A9Z04_RS24270 4985461..4985745 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
A9Z04_RS24275 4985745..4986014 - 270 WP_000605680.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A9Z04_RS24280 4986200..4987210 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
A9Z04_RS24285 4987344..4989041 - 1698 WP_000433464.1 malate dehydrogenase -
A9Z04_RS24290 4989198..4989335 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
A9Z04_RS24295 4989437..4989652 - 216 WP_000495766.1 biofilm-dependent modulation protein -
A9Z04_RS24300 4989997..4990428 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-89)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10181.73 Da        Isoelectric Point: 6.8582

>T64111 WP_000605680.1 NZ_CP016007:c4986014-4985745 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDA
YILDYEDYH

Download         Length: 270 bp

>T64111 NZ_CP016007:c4986014-4985745 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACACGATT
ACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTACATCCGTTAA
GCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAATGGCGATGCA
TACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT64111 WP_000781370.1 NZ_CP016007:c4985745-4985461 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT64111 NZ_CP016007:c4985745-4985461 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FUJ7

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References