Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3690413..3691107 Replicon chromosome
Accession NZ_CP016007
Organism Escherichia coli strain NGF1

Toxin (Protein)


Gene name yafO Uniprot ID B7MQ69
Locus tag A9Z04_RS17755 Protein ID WP_001263501.1
Coordinates 3690709..3691107 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag A9Z04_RS17750 Protein ID WP_000554755.1
Coordinates 3690413..3690706 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9Z04_RS17725 3685465..3685758 + 294 WP_000896590.1 hypothetical protein -
A9Z04_RS17730 3686127..3686699 + 573 WP_000086540.1 recombinase family protein -
A9Z04_RS17735 3686703..3687665 - 963 WP_000231497.1 hypothetical protein -
A9Z04_RS17740 3687703..3689247 - 1545 WP_000758648.1 recombinase family protein -
A9Z04_RS17745 3689309..3690361 + 1053 WP_000691316.1 DNA polymerase IV -
A9Z04_RS17750 3690413..3690706 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
A9Z04_RS17755 3690709..3691107 + 399 WP_001263501.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
A9Z04_RS17760 3691117..3691569 + 453 WP_001059897.1 GNAT family N-acetyltransferase -
A9Z04_RS17765 3691759..3692898 + 1140 WP_000521578.1 RNA ligase RtcB family protein -
A9Z04_RS17770 3692895..3693509 + 615 WP_000602123.1 peptide chain release factor H -
A9Z04_RS17775 3693566..3695023 - 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
A9Z04_RS17780 3695284..3695742 + 459 WP_001291991.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0950

>T64108 WP_001263501.1 NZ_CP016007:3690709-3691107 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T64108 NZ_CP016007:3690709-3691107 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT64108 WP_000554755.1 NZ_CP016007:3690413-3690706 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT64108 NZ_CP016007:3690413-3690706 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A376DBU4

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References