Detailed information of TA system TA064102

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2700097..2700699
• Replicon: chromosome
• Accession: NZ_CP016007
• Organism: Escherichia coli strain NGF1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: A9Z04_RS13035
• Protein ID: WP_000897302.1
• Coordinates: 2700097..2700408 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A9Z04_RS13040
• Protein ID: WP_000356397.1
• Coordinates: 2700409..2700699 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9Z04_RS13010	2696011..2696610	+	600	WP_001314324.1	glucose-1-phosphatase	-
A9Z04_RS13015	2696604..2697476	+	873	WP_000920762.1	virulence factor BrkB family protein	-
A9Z04_RS13020	2697473..2697910	+	438	WP_000560990.1	D-tyrosyl-tRNA(Tyr) deacylase	-
A9Z04_RS13025	2697955..2698896	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
A9Z04_RS13030	2698960..2699868	-	909	WP_001331553.1	alpha/beta hydrolase	-
A9Z04_RS13035	2700097..2700408	+	312	WP_000897302.1	hypothetical protein	Toxin
A9Z04_RS13040	2700409..2700699	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
A9Z04_RS13050	2701058..2701336	+	279	WP_001296612.1	hypothetical protein	-
A9Z04_RS13055	2701732..2701950	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
A9Z04_RS13060	2702166..2703095	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
A9Z04_RS13065	2703092..2703727	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
A9Z04_RS13070	2703724..2704626	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T64102 WP_000897302.1 NZ_CP016007:2700097-2700408 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T64102 NZ_CP016007:2700097-2700408 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT64102 WP_000356397.1 NZ_CP016007:2700409-2700699 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT64102 NZ_CP016007:2700409-2700699 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416