Detailed information of TA system TA064082

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 31046..31310
• Replicon: plasmid pS51_1
• Accession: NZ_CP015996
• Organism: Escherichia coli strain S51

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: A9K64_RS25195
• Protein ID: WP_001331364.1
• Coordinates: 31046..31198 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	31253..31310 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9K64_RS25165	26324..28486	+	2163	WP_000698351.1	DotA/TraY family protein	-
A9K64_RS25170	28551..29213	+	663	WP_000653334.1	plasmid IncI1-type surface exclusion protein ExcA	-
A9K64_RS25175	29285..29494	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
A9K64_RS29525	29886..30062	+	177	WP_001054904.1	hypothetical protein	-
A9K64_RS25190	30127..30423	-	297	WP_001275298.1	hypothetical protein	-
A9K64_RS25195	31046..31198	-	153	WP_001331364.1	Hok/Gef family protein	Toxin
-	31253..31310	+	58	NuclAT_0	-	Antitoxin
-	31253..31310	+	58	NuclAT_0	-	Antitoxin
-	31253..31310	+	58	NuclAT_0	-	Antitoxin
-	31253..31310	+	58	NuclAT_0	-	Antitoxin
A9K64_RS25200	31490..32698	+	1209	WP_000121274.1	IncI1-type conjugal transfer protein TrbA	-
A9K64_RS25205	32717..33787	+	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
A9K64_RS25210	33780..36071	+	2292	WP_001289276.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1A	-	1..93029	93029

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T64082 WP_001331364.1 NZ_CP015996:c31198-31046 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T64082 NZ_CP015996:c31198-31046 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT64082 NZ_CP015996:31253-31310 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin