Detailed information of TA system TA064081

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4791558..4792160
• Replicon: chromosome
• Accession: NZ_CP015995
• Organism: Escherichia coli strain S51

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: A9K64_RS24065
• Protein ID: WP_000897305.1
• Coordinates: 4791849..4792160 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A9K64_RS24060
• Protein ID: WP_000356397.1
• Coordinates: 4791558..4791848 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9K64_RS24025	4787505..4788407	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
A9K64_RS24030	4788404..4789039	+	636	WP_000829031.1	formate dehydrogenase cytochrome b556 subunit	-
A9K64_RS24035	4789036..4789965	+	930	WP_000027691.1	formate dehydrogenase accessory protein FdhE	-
A9K64_RS24045	4790295..4790537	-	243	WP_001086385.1	hypothetical protein	-
A9K64_RS24050	4790756..4790974	-	219	WP_001313440.1	ribbon-helix-helix domain-containing protein	-
A9K64_RS24060	4791558..4791848	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
A9K64_RS24065	4791849..4792160	-	312	WP_000897305.1	hypothetical protein	Toxin
A9K64_RS24070	4792389..4793297	+	909	WP_023155176.1	alpha/beta hydrolase	-
A9K64_RS24080	4793465..4795278	-	1814	Protein_4568	hypothetical protein	-
A9K64_RS24085	4795689..4796630	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
A9K64_RS24090	4796675..4797112	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12172.16 Da
• Isoelectric Point: 9.7791

>T64081 WP_000897305.1 NZ_CP015995:c4792160-4791849 [Escherichia coli]
ILFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T64081 NZ_CP015995:c4792160-4791849 [Escherichia coli]
ATACTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT64081 WP_000356397.1 NZ_CP015995:c4791848-4791558 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT64081 NZ_CP015995:c4791848-4791558 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7