Detailed information of TA system TA064047

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3493432..3493964
• Replicon: chromosome
• Accession: NZ_CP015992
• Organism: Pseudomonas sp. TCU-HL1

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: -
• Locus tag: THL1_RS16245
• Protein ID: WP_083245907.1
• Coordinates: 3493432..3493557 (+)
• Length: 42 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: A0A1B3E8H5
• Locus tag: THL1_RS16250
• Protein ID: WP_069084196.1
• Coordinates: 3493560..3493964 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THL1_RS16215	3489077..3489508	+	432	WP_069084193.1	phage tail protein	-
THL1_RS16220	3489505..3490512	+	1008	WP_069084194.1	phage late control D family protein	-
THL1_RS16225	3490806..3491048	+	243	WP_069084195.1	hypothetical protein	-
THL1_RS16230	3491250..3491408	+	159	WP_145928312.1	type II toxin-antitoxin system MqsR family toxin	-
THL1_RS16235	3491490..3491843	+	354	WP_096368068.1	IS66 family insertion sequence element accessory protein TnpB	-
THL1_RS16240	3491863..3493362	+	1500	WP_083245827.1	IS66 family transposase	-
THL1_RS16245	3493432..3493557	+	126	WP_083245907.1	type II toxin-antitoxin system MqsR family toxin	Toxin
THL1_RS16250	3493560..3493964	+	405	WP_069084196.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
THL1_RS16255	3494141..3495049	-	909	WP_069084197.1	DUF2806 domain-containing protein	-
THL1_RS16260	3495097..3495585	-	489	WP_083245908.1	GIY-YIG nuclease family protein	-
THL1_RS29725	3495637..3496203	-	567	WP_145928313.1	hypothetical protein	-
THL1_RS16270	3496200..3497129	-	930	WP_069084200.1	hypothetical protein	-
THL1_RS16275	3497172..3497573	-	402	WP_069084201.1	hypothetical protein	-
THL1_RS16280	3497641..3498006	-	366	WP_069084202.1	helix-turn-helix domain-containing protein	-
THL1_RS16285	3498091..3498330	+	240	WP_069084203.1	DNA-binding protein	-
THL1_RS16290	3498343..3498531	+	189	WP_069084204.1	hypothetical protein	-
THL1_RS16295	3498560..3498742	+	183	WP_069084205.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3440739..3537185	96446

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (1-40)

Antitoxin

• Zinc finger, MqsA-type (3-44)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4878.61 Da
• Isoelectric Point: 4.3527

>T64047 WP_083245907.1 NZ_CP015992:3493432-3493557 [Pseudomonas sp. TCU-HL1]
MTTYADHTIWQDVYRPTTEVGQLYLKFTLVEDLLIVSFKEL

Nucleotide

• Length: 126 bp

>T64047 NZ_CP015992:3493432-3493557 [Pseudomonas sp. TCU-HL1]
ATGACCACCTACGCCGACCACACCATCTGGCAGGACGTTTACCGCCCCACCACCGAGGTCGGCCAGCTCTACCTCAAGTT
CACCCTGGTGGAGGACCTTCTCATCGTGTCCTTCAAGGAGCTCTGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14752.05 Da
• Isoelectric Point: 8.4953

>AT64047 WP_069084196.1 NZ_CP015992:3493560-3493964 [Pseudomonas sp. TCU-HL1]
MKCPVCGQADLVRDVRDMPYIYKGEATLIPNVEADWCNACGESLTGPAESKRVMLAMNLARQQVNARAGHLELIRAVRKQ
LKLTQKQAGEIFGGGPNAFSRYEKGDVDAPTALVKLFQLLQKHPELGDEVKRTG

Nucleotide

• Length: 405 bp

>AT64047 NZ_CP015992:3493560-3493964 [Pseudomonas sp. TCU-HL1]
ATGAAATGCCCGGTATGCGGCCAGGCCGACCTGGTCCGCGACGTTCGCGACATGCCGTACATCTACAAGGGCGAGGCCAC
CCTCATCCCCAACGTAGAGGCGGACTGGTGCAACGCCTGCGGAGAGTCCCTCACAGGTCCGGCGGAATCCAAACGAGTCA
TGCTGGCAATGAACCTTGCCCGGCAGCAGGTCAACGCACGCGCCGGGCACCTGGAGCTGATCAGAGCAGTACGCAAGCAG
CTCAAACTCACCCAGAAGCAGGCGGGCGAGATCTTCGGTGGCGGCCCCAATGCATTCAGTCGCTACGAGAAAGGCGATGT
GGATGCGCCAACAGCGCTGGTGAAACTGTTCCAGTTGCTGCAGAAGCATCCCGAGTTGGGGGATGAGGTGAAGCGTACCG
GTTGA

Structures

Antitoxin

• AlphaFold DB: A0A1B3E8H5