Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3972550..3973147 Replicon chromosome
Accession NZ_CP015990
Organism Klebsiella pneumoniae strain BR

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag A9J37_RS19470 Protein ID WP_004142563.1
Coordinates 3972830..3973147 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag A9J37_RS19465 Protein ID WP_004142561.1
Coordinates 3972550..3972837 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9J37_RS19435 3968630..3968878 + 249 WP_020325243.1 DUF1158 domain-containing protein -
A9J37_RS19440 3968896..3969237 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
A9J37_RS19445 3969268..3970383 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
A9J37_RS19450 3970563..3971144 + 582 WP_064820848.1 TetR/AcrR family transcriptional regulator -
A9J37_RS19455 3971144..3971512 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
A9J37_RS19460 3971632..3972285 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
A9J37_RS19465 3972550..3972837 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
A9J37_RS19470 3972830..3973147 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A9J37_RS19475 3973332..3974375 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
A9J37_RS19480 3975045..3975911 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
A9J37_RS19485 3976020..3977447 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T64038 WP_004142563.1 NZ_CP015990:c3973147-3972830 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T64038 NZ_CP015990:c3973147-3972830 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT64038 WP_004142561.1 NZ_CP015990:c3972837-3972550 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT64038 NZ_CP015990:c3972837-3972550 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References