Detailed information of TA system TA063988

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 13699..14359
• Replicon: plasmid unnamed1
• Accession: NZ_CP015957
• Organism: Legionella pneumophila strain E9_O

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2Z3KTA1
• Locus tag: A9E96_RS15005
• Protein ID: WP_019232992.1
• Coordinates: 13997..14359 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2Z3KXZ6
• Locus tag: A9E96_RS15000
• Protein ID: WP_019232993.1
• Coordinates: 13699..14004 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9E96_RS14985	9052..9663	-	612	WP_019232996.1	hypothetical protein	-
A9E96_RS14990	9674..12343	-	2670	WP_019232995.1	Ti-type conjugative transfer relaxase TraA	-
A9E96_RS14995	12569..12865	+	297	WP_019232994.1	hypothetical protein	-
A9E96_RS15725	12881..13138	+	258	WP_127908205.1	hypothetical protein	-
A9E96_RS15615	13150..13386	+	237	WP_080480132.1	hypothetical protein	-
A9E96_RS15000	13699..14004	-	306	WP_019232993.1	helix-turn-helix domain-containing protein	Antitoxin
A9E96_RS15005	13997..14359	-	363	WP_019232992.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A9E96_RS15010	14523..15845	+	1323	WP_019232991.1	DUF1186 domain-containing protein	-
A9E96_RS15015	15860..16264	-	405	WP_019232990.1	type II toxin-antitoxin system HicB family antitoxin	-
A9E96_RS15020	16261..16458	-	198	WP_019232989.1	type II toxin-antitoxin system HicA family toxin	-
A9E96_RS15025	16628..16954	-	327	WP_019232988.1	hypothetical protein	-
A9E96_RS15030	16978..17832	-	855	WP_080480134.1	site-specific integrase	-
A9E96_RS15730	17836..17997	-	162	Protein_21	site-specific integrase	-
A9E96_RS15035	18088..18315	-	228	WP_019232986.1	ChaB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..48354	48354

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (47-110)

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-88)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13438.39 Da
• Isoelectric Point: 9.9492

>T63988 WP_019232992.1 NZ_CP015957:c14359-13997 [Legionella pneumophila]
MKRMITIIEFPGFLSQVGNSISAAERDEFINFIARNPEVGDLIPGTGGVRKVRWGSKNKGKSGGVRVIYYFYDELAPVFL
LTAYGKGEKENLTAEQKKQISALAQVLKSECKNRRSQEHD

Nucleotide

• Length: 363 bp

>T63988 NZ_CP015957:c14359-13997 [Legionella pneumophila]
ATGAAAAGAATGATAACAATAATCGAATTTCCTGGGTTTCTTTCTCAAGTTGGAAACTCAATTAGCGCGGCCGAACGCGA
TGAATTTATTAATTTTATCGCCAGAAATCCAGAGGTTGGGGATCTAATCCCTGGAACTGGTGGAGTGAGAAAAGTGCGTT
GGGGAAGTAAGAATAAGGGAAAAAGCGGTGGCGTCAGGGTAATTTATTATTTTTACGATGAGCTTGCCCCTGTTTTTTTG
TTAACTGCTTATGGAAAAGGTGAAAAAGAGAATTTAACTGCCGAGCAAAAGAAACAAATATCAGCTCTTGCTCAAGTTTT
AAAGTCTGAGTGCAAGAACCGTAGGAGTCAAGAACATGACTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11531.14 Da
• Isoelectric Point: 8.6768

>AT63988 WP_019232993.1 NZ_CP015957:c14004-13699 [Legionella pneumophila]
MTNRNIGNEIIQGMEEAIEYIRGNKGSAKVHKVEIPNEIDVREIRENLNLSRKEFADSFGFSPRTLQHWEQGDRAPHGAA
KVLLLLLQREPATIAKILRQN

Nucleotide

• Length: 306 bp

>AT63988 NZ_CP015957:c14004-13699 [Legionella pneumophila]
ATGACTAATCGAAATATAGGAAATGAAATAATTCAAGGCATGGAAGAGGCTATTGAATATATTCGAGGGAATAAAGGATC
TGCAAAGGTTCATAAAGTTGAAATACCCAATGAAATTGATGTTCGGGAAATTCGAGAGAATCTTAATTTATCCCGAAAAG
AATTCGCAGACAGTTTTGGCTTTAGTCCGAGAACGTTGCAGCATTGGGAGCAGGGGGATAGAGCCCCGCATGGCGCAGCT
AAAGTCCTGTTGTTGCTTTTGCAGCGAGAGCCAGCAACTATTGCTAAAATTTTACGCCAAAATTGA

Structures

Toxin

• AlphaFold DB: A0A2Z3KTA1

Antitoxin

• AlphaFold DB: A0A2Z3KXZ6