Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 8224..8963 Replicon plasmid unnamed1
Accession NZ_CP015957
Organism Legionella pneumophila strain E9_O

Toxin (Protein)


Gene name higB Uniprot ID A0A2Z3KTI3
Locus tag A9E96_RS14980 Protein ID WP_019232997.1
Coordinates 8652..8963 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2Z3L5D3
Locus tag A9E96_RS14975 Protein ID WP_019232998.1
Coordinates 8224..8655 (-) Length 144 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9E96_RS14965 5564..6322 + 759 WP_085956434.1 hypothetical protein -
A9E96_RS14970 6322..8187 + 1866 WP_019232999.1 type IV conjugative transfer system coupling protein TraD -
A9E96_RS14975 8224..8655 - 432 WP_019232998.1 transcriptional regulator Antitoxin
A9E96_RS14980 8652..8963 - 312 WP_019232997.1 type II toxin-antitoxin system HigB family toxin Toxin
A9E96_RS14985 9052..9663 - 612 WP_019232996.1 hypothetical protein -
A9E96_RS14990 9674..12343 - 2670 WP_019232995.1 Ti-type conjugative transfer relaxase TraA -
A9E96_RS14995 12569..12865 + 297 WP_019232994.1 hypothetical protein -
A9E96_RS15725 12881..13138 + 258 WP_127908205.1 hypothetical protein -
A9E96_RS15615 13150..13386 + 237 WP_080480132.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..48354 48354


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Antitoxin HigA

(1-143)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.55 Da        Isoelectric Point: 10.2563

>T63987 WP_019232997.1 NZ_CP015957:c8963-8652 [Legionella pneumophila]
MHVISKKPFVEAAKKYPNQRQAILDLYRVLDKITVNTPLEMKAIFPSLDNFKYKDKWWIIDIGGNHLRLMAFIQFSQNRI
YVKHIVTHAEYDRLCEQYRRNKQ

Download         Length: 312 bp

>T63987 NZ_CP015957:c8963-8652 [Legionella pneumophila]
ATGCATGTTATCTCTAAAAAACCATTTGTTGAGGCTGCTAAGAAATATCCGAATCAACGTCAGGCGATTTTAGATTTATA
TCGTGTGCTAGACAAGATAACAGTGAATACCCCATTGGAAATGAAAGCAATTTTTCCAAGCCTTGATAACTTTAAGTATA
AAGATAAGTGGTGGATTATTGATATAGGAGGTAATCATTTACGACTGATGGCTTTTATTCAATTTTCACAAAATAGAATC
TATGTGAAACATATTGTCACTCATGCTGAATACGATAGATTATGTGAGCAGTACAGGAGGAATAAACAATGA

Antitoxin


Download         Length: 144 a.a.        Molecular weight: 16294.65 Da        Isoelectric Point: 4.7426

>AT63987 WP_019232998.1 NZ_CP015957:c8655-8224 [Legionella pneumophila]
MISEQVILNKFIDFTKVAAPVLSLKDEQAYEDALTMMEHLMESVGEQRNKPENILIGLLAHAIEEYESKDEEISDFVSQS
MSDRGDVALLRMIIDQHQLTLSDLPEVGHKSLISKILSGERNLTRSHIEKLSKRFHIDPGLFF

Download         Length: 432 bp

>AT63987 NZ_CP015957:c8655-8224 [Legionella pneumophila]
ATGATTTCTGAACAGGTTATTTTAAACAAATTTATTGATTTCACTAAAGTTGCAGCTCCTGTTCTTTCTTTAAAAGATGA
ACAGGCTTATGAAGATGCTTTGACGATGATGGAGCATTTAATGGAATCTGTTGGTGAGCAACGTAATAAACCCGAAAATA
TATTAATTGGATTGTTGGCGCACGCAATAGAAGAATATGAGTCTAAAGATGAAGAAATTTCTGATTTTGTGAGTCAGTCT
ATGTCAGATCGGGGCGATGTTGCCCTACTCAGAATGATTATCGATCAGCACCAATTAACACTCAGTGATTTACCTGAGGT
AGGGCATAAGTCTCTCATATCAAAAATATTATCTGGGGAGCGAAATCTTACTCGATCACACATCGAAAAATTAAGTAAAC
GGTTTCATATTGACCCTGGGTTGTTTTTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z3KTI3

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3L5D3

References