Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) HipBST/HipA(toxin)
Location 2702509..2703752 Replicon chromosome
Accession NZ_CP015954
Organism Legionella pneumophila strain E7_O

Toxin (Protein)


Gene name HipT Uniprot ID Q5ZSZ6
Locus tag A9E94_RS11945 Protein ID WP_010948074.1
Coordinates 2702814..2703752 (+) Length 313 a.a.

Antitoxin (Protein)


Gene name HipS Uniprot ID Q5ZSZ7
Locus tag A9E94_RS11940 Protein ID WP_010948073.1
Coordinates 2702509..2702817 (+) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9E94_RS11915 2697945..2698310 + 366 WP_010948069.1 BRCT domain-containing protein -
A9E94_RS11920 2698667..2699032 - 366 WP_010948070.1 TraK family protein -
A9E94_RS15840 2699217..2699357 - 141 WP_153802426.1 hypothetical protein -
A9E94_RS11925 2699360..2701111 - 1752 WP_010948071.1 DUF927 domain-containing protein -
A9E94_RS11930 2701177..2701452 - 276 WP_010948072.1 helix-turn-helix domain-containing protein -
A9E94_RS11935 2702285..2702512 + 228 WP_006870829.1 helix-turn-helix transcriptional regulator -
A9E94_RS11940 2702509..2702817 + 309 WP_010948073.1 HipA N-terminal domain-containing protein Antitoxin
A9E94_RS11945 2702814..2703752 + 939 WP_010948074.1 lpg2370 family Dot/Icm T4SS effector Toxin
A9E94_RS11950 2704295..2704909 + 615 WP_010948075.1 hypothetical protein -
A9E94_RS11955 2705065..2705271 - 207 WP_015444115.1 hypothetical protein -
A9E94_RS11960 2705608..2706876 + 1269 WP_010948076.1 lpg2372 family Dot/Icm T4SS effector -
A9E94_RS11965 2707092..2707617 - 526 Protein_2356 hypothetical protein -
A9E94_RS11970 2707617..2708282 - 666 WP_015444114.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2696531..2697706 1175


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(53-276)

Antitoxin

HipA, N-terminal subdomain 1

(5-98)


Sequences

Toxin        


Download         Length: 313 a.a.        Molecular weight: 36077.81 Da        Isoelectric Point: 8.6882

>T63983 WP_010948074.1 NZ_CP015954:2702814-2703752 [Legionella pneumophila]
MKHCPITYEKISDQENYSQRGLHLLSPQLKNLSPLDLSADEQRQEAIARVGKMSVQGVQKKLSAKLKIKEGCFEIVDQYG
QYILKPQSDIYPELPENEAITMTLAKTIGLEVPVHGLVYSKDNSLTYFIKRFDRIGHNKKLALEDFAQLSGEDRHTKYKS
SMEKVIAVIEQFCTFPKIEFVKLFKLTLFNFLVGNEDMHLKNFSLITKDRKISISPAYDLLNSTIAQKNTKEELALPLKG
KKNNLTKSDFLKYFAIEKLGLNQNVIDGIVQEFHQVIPKWQELIGFSFLSQEMQEKYLELLEQRCKRLNFFD

Download         Length: 939 bp

>T63983 NZ_CP015954:2702814-2703752 [Legionella pneumophila]
ATGAAACACTGCCCTATTACTTATGAAAAGATTAGTGACCAGGAAAACTATTCGCAACGTGGATTGCATTTGCTTTCTCC
TCAATTAAAAAACCTTAGTCCATTGGATTTAAGTGCTGATGAGCAGCGTCAGGAAGCAATTGCTCGAGTAGGAAAGATGT
CTGTTCAAGGCGTCCAAAAGAAACTAAGCGCAAAACTAAAGATTAAAGAAGGCTGTTTTGAAATCGTGGATCAATATGGT
CAGTATATTTTAAAACCACAAAGTGATATTTATCCAGAATTGCCTGAAAATGAAGCCATTACTATGACCCTTGCAAAAAC
TATAGGCCTCGAAGTTCCGGTTCATGGTTTGGTTTATTCTAAAGACAACAGTTTAACCTACTTCATTAAACGCTTTGATA
GAATAGGCCATAATAAAAAGTTAGCTTTAGAAGATTTTGCACAGCTATCAGGTGAAGATCGACATACAAAATATAAAAGT
TCTATGGAAAAAGTAATTGCAGTCATAGAACAGTTTTGTACATTCCCAAAAATTGAATTCGTGAAATTATTTAAGTTGAC
GTTGTTTAACTTCTTGGTCGGTAATGAAGACATGCATCTAAAAAACTTTTCCTTAATTACGAAGGATAGAAAAATTTCCA
TATCACCAGCCTACGATTTACTTAACTCAACCATCGCTCAAAAAAACACAAAAGAAGAACTGGCTTTACCTCTAAAAGGA
AAAAAAAATAATTTAACAAAGAGTGATTTTCTTAAATACTTTGCCATTGAAAAATTAGGATTAAACCAGAACGTCATCGA
TGGGATCGTACAAGAGTTCCATCAGGTAATACCAAAATGGCAAGAGCTGATTGGCTTCAGTTTTTTATCTCAAGAGATGC
AGGAAAAATATCTCGAATTGCTAGAGCAACGATGCAAGCGATTAAATTTTTTTGATTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11557.38 Da        Isoelectric Point: 4.5994

>AT63983 WP_010948073.1 NZ_CP015954:2702509-2702817 [Legionella pneumophila]
MRKAYVSVSGIKAGILEELQGGTYQFTYFEDYHGAPVSLTMPLKNKVYDFDVFPPFFEGLLPEGIMLEALLRKYKIDKND
YFGQLILVGQDVVGAVTIEEIR

Download         Length: 309 bp

>AT63983 NZ_CP015954:2702509-2702817 [Legionella pneumophila]
ATGAGAAAAGCATACGTATCGGTGAGTGGCATTAAGGCTGGAATATTAGAAGAATTACAAGGTGGAACCTATCAATTTAC
CTACTTTGAGGATTATCACGGCGCACCTGTTTCACTCACTATGCCGTTGAAAAATAAGGTCTATGATTTTGACGTGTTCC
CCCCTTTTTTTGAAGGATTACTTCCTGAAGGCATTATGCTTGAAGCACTATTACGCAAATACAAGATAGATAAAAATGAT
TATTTTGGTCAATTGATACTAGTTGGCCAAGATGTCGTAGGCGCAGTGACTATTGAGGAAATAAGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VKC
PDB 7VKB
PDB 7WCF

Antitoxin

Source ID Structure
PDB 7VKB
AlphaFold DB Q5ZSZ7

References