63974

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) HipBST/HipA(toxin)
Location 2741869..2743112 Replicon chromosome
Accession NZ_CP015949
Organism Legionella pneumophila strain E3_N

Toxin (Protein)


Gene name HipT Uniprot ID Q5ZSZ6
Locus tag A9E90_RS12155 Protein ID WP_010948074.1
Coordinates 2742174..2743112 (+) Length 313 a.a.

Antitoxin (Protein)


Gene name HipS Uniprot ID Q5ZSZ7
Locus tag A9E90_RS12150 Protein ID WP_010948073.1
Coordinates 2741869..2742177 (+) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9E90_RS12125 2737305..2737670 + 366 WP_010948069.1 BRCT domain-containing protein -
A9E90_RS12130 2738027..2738392 - 366 WP_010948070.1 TraK family protein -
A9E90_RS16035 2738577..2738717 - 141 WP_153802426.1 hypothetical protein -
A9E90_RS12135 2738720..2740471 - 1752 WP_010948071.1 DUF927 domain-containing protein -
A9E90_RS12140 2740537..2740812 - 276 WP_010948072.1 helix-turn-helix domain-containing protein -
A9E90_RS12145 2741645..2741872 + 228 WP_006870829.1 helix-turn-helix transcriptional regulator -
A9E90_RS12150 2741869..2742177 + 309 WP_010948073.1 HipA N-terminal domain-containing protein Antitoxin
A9E90_RS12155 2742174..2743112 + 939 WP_010948074.1 lpg2370 family Dot/Icm T4SS effector Toxin
A9E90_RS12160 2743655..2744269 + 615 WP_010948075.1 hypothetical protein -
A9E90_RS12165 2744425..2744631 - 207 WP_015444115.1 hypothetical protein -
A9E90_RS12170 2744968..2746236 + 1269 WP_010948076.1 lpg2372 family Dot/Icm T4SS effector -
A9E90_RS12175 2746452..2746977 - 526 Protein_2396 hypothetical protein -
A9E90_RS12180 2746977..2747642 - 666 WP_015444114.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2735891..2737066 1175


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(53-276)

Antitoxin

HipA, N-terminal subdomain 1

(5-98)


Sequences

Toxin        


Download         Length: 313 a.a.        Molecular weight: 36077.81 Da        Isoelectric Point: 8.6882

>T63974 WP_010948074.1 NZ_CP015949:2742174-2743112 [Legionella pneumophila]
MKHCPITYEKISDQENYSQRGLHLLSPQLKNLSPLDLSADEQRQEAIARVGKMSVQGVQKKLSAKLKIKEGCFEIVDQYG
QYILKPQSDIYPELPENEAITMTLAKTIGLEVPVHGLVYSKDNSLTYFIKRFDRIGHNKKLALEDFAQLSGEDRHTKYKS
SMEKVIAVIEQFCTFPKIEFVKLFKLTLFNFLVGNEDMHLKNFSLITKDRKISISPAYDLLNSTIAQKNTKEELALPLKG
KKNNLTKSDFLKYFAIEKLGLNQNVIDGIVQEFHQVIPKWQELIGFSFLSQEMQEKYLELLEQRCKRLNFFD

Download         Length: 939 bp

>T63974 NZ_CP015949:2742174-2743112 [Legionella pneumophila]
ATGAAACACTGCCCTATTACTTATGAAAAGATTAGTGACCAGGAAAACTATTCGCAACGTGGATTGCATTTGCTTTCTCC
TCAATTAAAAAACCTTAGTCCATTGGATTTAAGTGCTGATGAGCAGCGTCAGGAAGCAATTGCTCGAGTAGGAAAGATGT
CTGTTCAAGGCGTCCAAAAGAAACTAAGCGCAAAACTAAAGATTAAAGAAGGCTGTTTTGAAATCGTGGATCAATATGGT
CAGTATATTTTAAAACCACAAAGTGATATTTATCCAGAATTGCCTGAAAATGAAGCCATTACTATGACCCTTGCAAAAAC
TATAGGCCTCGAAGTTCCGGTTCATGGTTTGGTTTATTCTAAAGACAACAGTTTAACCTACTTCATTAAACGCTTTGATA
GAATAGGCCATAATAAAAAGTTAGCTTTAGAAGATTTTGCACAGCTATCAGGTGAAGATCGACATACAAAATATAAAAGT
TCTATGGAAAAAGTAATTGCAGTCATAGAACAGTTTTGTACATTCCCAAAAATTGAATTCGTGAAATTATTTAAGTTGAC
GTTGTTTAACTTCTTGGTCGGTAATGAAGACATGCATCTAAAAAACTTTTCCTTAATTACGAAGGATAGAAAAATTTCCA
TATCACCAGCCTACGATTTACTTAACTCAACCATCGCTCAAAAAAACACAAAAGAAGAACTGGCTTTACCTCTAAAAGGA
AAAAAAAATAATTTAACAAAGAGTGATTTTCTTAAATACTTTGCCATTGAAAAATTAGGATTAAACCAGAACGTCATCGA
TGGGATCGTACAAGAGTTCCATCAGGTAATACCAAAATGGCAAGAGCTGATTGGCTTCAGTTTTTTATCTCAAGAGATGC
AGGAAAAATATCTCGAATTGCTAGAGCAACGATGCAAGCGATTAAATTTTTTTGATTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11557.38 Da        Isoelectric Point: 4.5994

>AT63974 WP_010948073.1 NZ_CP015949:2741869-2742177 [Legionella pneumophila]
MRKAYVSVSGIKAGILEELQGGTYQFTYFEDYHGAPVSLTMPLKNKVYDFDVFPPFFEGLLPEGIMLEALLRKYKIDKND
YFGQLILVGQDVVGAVTIEEIR

Download         Length: 309 bp

>AT63974 NZ_CP015949:2741869-2742177 [Legionella pneumophila]
ATGAGAAAAGCATACGTATCGGTGAGTGGCATTAAGGCTGGAATATTAGAAGAATTACAAGGTGGAACCTATCAATTTAC
CTACTTTGAGGATTATCACGGCGCACCTGTTTCACTCACTATGCCGTTGAAAAATAAGGTCTATGATTTTGACGTGTTCC
CCCCTTTTTTTGAAGGATTACTTCCTGAAGGCATTATGCTTGAAGCACTATTACGCAAATACAAGATAGATAAAAATGAT
TATTTTGGTCAATTGATACTAGTTGGCCAAGATGTCGTAGGCGCAGTGACTATTGAGGAAATAAGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VKC
PDB 7VKB
PDB 7WCF

Antitoxin

Source ID Structure
PDB 7VKB
AlphaFold DB Q5ZSZ7

References