63971

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) HipBST/HipA(toxin)
Location 2613466..2614709 Replicon chromosome
Accession NZ_CP015947
Organism Legionella pneumophila strain E2_N

Toxin (Protein)


Gene name HipT Uniprot ID Q5ZSZ6
Locus tag A9E89_RS11630 Protein ID WP_010948074.1
Coordinates 2613771..2614709 (+) Length 313 a.a.

Antitoxin (Protein)


Gene name HipS Uniprot ID Q5ZSZ7
Locus tag A9E89_RS11625 Protein ID WP_010948073.1
Coordinates 2613466..2613774 (+) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9E89_RS11600 2608902..2609267 + 366 WP_010948069.1 BRCT domain-containing protein -
A9E89_RS11605 2609624..2609989 - 366 WP_010948070.1 TraK family protein -
A9E89_RS15835 2610174..2610314 - 141 WP_153802426.1 hypothetical protein -
A9E89_RS11610 2610317..2612068 - 1752 WP_010948071.1 DUF927 domain-containing protein -
A9E89_RS11615 2612134..2612409 - 276 WP_010948072.1 helix-turn-helix domain-containing protein -
A9E89_RS11620 2613242..2613469 + 228 WP_006870829.1 helix-turn-helix transcriptional regulator -
A9E89_RS11625 2613466..2613774 + 309 WP_010948073.1 HipA N-terminal domain-containing protein Antitoxin
A9E89_RS11630 2613771..2614709 + 939 WP_010948074.1 lpg2370 family Dot/Icm T4SS effector Toxin
A9E89_RS11635 2615252..2615866 + 615 WP_010948075.1 hypothetical protein -
A9E89_RS11640 2616022..2616228 - 207 WP_015444115.1 hypothetical protein -
A9E89_RS11645 2616565..2617833 + 1269 WP_010948076.1 lpg2372 family Dot/Icm T4SS effector -
A9E89_RS11650 2618049..2618574 - 526 Protein_2288 hypothetical protein -
A9E89_RS11655 2618574..2619239 - 666 WP_015444114.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2607488..2608663 1175


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(53-276)

Antitoxin

HipA, N-terminal subdomain 1

(5-98)


Sequences

Toxin        


Download         Length: 313 a.a.        Molecular weight: 36077.81 Da        Isoelectric Point: 8.6882

>T63971 WP_010948074.1 NZ_CP015947:2613771-2614709 [Legionella pneumophila]
MKHCPITYEKISDQENYSQRGLHLLSPQLKNLSPLDLSADEQRQEAIARVGKMSVQGVQKKLSAKLKIKEGCFEIVDQYG
QYILKPQSDIYPELPENEAITMTLAKTIGLEVPVHGLVYSKDNSLTYFIKRFDRIGHNKKLALEDFAQLSGEDRHTKYKS
SMEKVIAVIEQFCTFPKIEFVKLFKLTLFNFLVGNEDMHLKNFSLITKDRKISISPAYDLLNSTIAQKNTKEELALPLKG
KKNNLTKSDFLKYFAIEKLGLNQNVIDGIVQEFHQVIPKWQELIGFSFLSQEMQEKYLELLEQRCKRLNFFD

Download         Length: 939 bp

>T63971 NZ_CP015947:2613771-2614709 [Legionella pneumophila]
ATGAAACACTGCCCTATTACTTATGAAAAGATTAGTGACCAGGAAAACTATTCGCAACGTGGATTGCATTTGCTTTCTCC
TCAATTAAAAAACCTTAGTCCATTGGATTTAAGTGCTGATGAGCAGCGTCAGGAAGCAATTGCTCGAGTAGGAAAGATGT
CTGTTCAAGGCGTCCAAAAGAAACTAAGCGCAAAACTAAAGATTAAAGAAGGCTGTTTTGAAATCGTGGATCAATATGGT
CAGTATATTTTAAAACCACAAAGTGATATTTATCCAGAATTGCCTGAAAATGAAGCCATTACTATGACCCTTGCAAAAAC
TATAGGCCTCGAAGTTCCGGTTCATGGTTTGGTTTATTCTAAAGACAACAGTTTAACCTACTTCATTAAACGCTTTGATA
GAATAGGCCATAATAAAAAGTTAGCTTTAGAAGATTTTGCACAGCTATCAGGTGAAGATCGACATACAAAATATAAAAGT
TCTATGGAAAAAGTAATTGCAGTCATAGAACAGTTTTGTACATTCCCAAAAATTGAATTCGTGAAATTATTTAAGTTGAC
GTTGTTTAACTTCTTGGTCGGTAATGAAGACATGCATCTAAAAAACTTTTCCTTAATTACGAAGGATAGAAAAATTTCCA
TATCACCAGCCTACGATTTACTTAACTCAACCATCGCTCAAAAAAACACAAAAGAAGAACTGGCTTTACCTCTAAAAGGA
AAAAAAAATAATTTAACAAAGAGTGATTTTCTTAAATACTTTGCCATTGAAAAATTAGGATTAAACCAGAACGTCATCGA
TGGGATCGTACAAGAGTTCCATCAGGTAATACCAAAATGGCAAGAGCTGATTGGCTTCAGTTTTTTATCTCAAGAGATGC
AGGAAAAATATCTCGAATTGCTAGAGCAACGATGCAAGCGATTAAATTTTTTTGATTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11557.38 Da        Isoelectric Point: 4.5994

>AT63971 WP_010948073.1 NZ_CP015947:2613466-2613774 [Legionella pneumophila]
MRKAYVSVSGIKAGILEELQGGTYQFTYFEDYHGAPVSLTMPLKNKVYDFDVFPPFFEGLLPEGIMLEALLRKYKIDKND
YFGQLILVGQDVVGAVTIEEIR

Download         Length: 309 bp

>AT63971 NZ_CP015947:2613466-2613774 [Legionella pneumophila]
ATGAGAAAAGCATACGTATCGGTGAGTGGCATTAAGGCTGGAATATTAGAAGAATTACAAGGTGGAACCTATCAATTTAC
CTACTTTGAGGATTATCACGGCGCACCTGTTTCACTCACTATGCCGTTGAAAAATAAGGTCTATGATTTTGACGTGTTCC
CCCCTTTTTTTGAAGGATTACTTCCTGAAGGCATTATGCTTGAAGCACTATTACGCAAATACAAGATAGATAAAAATGAT
TATTTTGGTCAATTGATACTAGTTGGCCAAGATGTCGTAGGCGCAGTGACTATTGAGGAAATAAGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VKC
PDB 7VKB
PDB 7WCF

Antitoxin

Source ID Structure
AlphaFold DB Q5ZSZ7

References