Detailed information of TA system TA063932

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 12107..12371
• Replicon: plasmid pSLy4
• Accession: NZ_CP015916
• Organism: Escherichia coli strain 210205630 isolate 210205630

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: A9C00_RS26615
• Protein ID: WP_001303307.1
• Coordinates: 12219..12371 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	12107..12169 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9C00_RS26600	7346..9637	-	2292	WP_001289269.1	TrbC	-
A9C00_RS26605	9630..10700	-	1071	WP_000151576.1	IncI1-type conjugal transfer protein TrbB	-
A9C00_RS26610	10719..11927	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	12107..12169	-	63	NuclAT_0	-	Antitoxin
-	12107..12169	-	63	NuclAT_0	-	Antitoxin
-	12107..12169	-	63	NuclAT_0	-	Antitoxin
-	12107..12169	-	63	NuclAT_0	-	Antitoxin
A9C00_RS26615	12219..12371	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
A9C00_RS26620	12443..12694	-	252	WP_001291965.1	hypothetical protein	-
-	13081..13132	-	52	NuclAT_1	-	-
-	13081..13132	-	52	NuclAT_1	-	-
-	13081..13132	-	52	NuclAT_1	-	-
-	13081..13132	-	52	NuclAT_1	-	-
A9C00_RS31030	13618..13794	-	177	WP_001054900.1	hypothetical protein	-
A9C00_RS26635	14186..14395	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
A9C00_RS26640	14467..15129	-	663	WP_000644794.1	plasmid IncI1-type surface exclusion protein ExcA	-
A9C00_RS26645	15200..17368	-	2169	WP_000698368.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..100096	100096

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T63932 WP_001303307.1 NZ_CP015916:12219-12371 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T63932 NZ_CP015916:12219-12371 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT63932 NZ_CP015916:c12169-12107 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin