Detailed information of TA system TA063926

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 49606..50032
• Replicon: plasmid pSLy2
• Accession: NZ_CP015914
• Organism: Escherichia coli strain 210205630 isolate 210205630

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: A9C00_RS25495
• Protein ID: WP_001312861.1
• Coordinates: 49606..49764 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	49808..50032 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9C00_RS25475	44606..44890	-	285	WP_065304459.1	hypothetical protein	-
A9C00_RS25480	44894..47860	-	2967	WP_000008826.1	Tn3 family transposase	-
A9C00_RS25485	47841..48463	-	623	Protein_49	recombinase family protein	-
A9C00_RS25495	49606..49764	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	49808..50032	-	225	NuclAT_0	-	Antitoxin
-	49808..50032	-	225	NuclAT_0	-	Antitoxin
-	49808..50032	-	225	NuclAT_0	-	Antitoxin
-	49808..50032	-	225	NuclAT_0	-	Antitoxin
A9C00_RS30835	49844..50032	+	189	WP_001299721.1	hypothetical protein	-
A9C00_RS25505	50044..50763	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
A9C00_RS25510	50760..51194	-	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
A9C00_RS25515	51249..53207	-	1959	WP_065304460.1	ParB/RepB/Spo0J family partition protein	-
A9C00_RS25520	53272..53505	-	234	WP_065304461.1	DUF905 domain-containing protein	-
A9C00_RS25525	53563..54102	-	540	WP_065304462.1	single-stranded DNA-binding protein	-
A9C00_RS31020	54128..54334	-	207	WP_000275853.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	oqxB / oqxA / blaTEM-1B	-	1..129035	129035
	-	flank	IS/Tn	-	-	48023..48463	440

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T63926 WP_001312861.1 NZ_CP015914:c49764-49606 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T63926 NZ_CP015914:c49764-49606 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT63926 NZ_CP015914:c50032-49808 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin