Detailed information of TA system TA063922

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 10534..10788
• Replicon: plasmid pSLy2
• Accession: NZ_CP015914
• Organism: Escherichia coli strain 210205630 isolate 210205630

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: A9C00_RS25305
• Protein ID: WP_001351576.1
• Coordinates: 10534..10740 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	10727..10788 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9C00_RS25275	6049..7277	+	1229	WP_113706755.1	IS3 family transposase	-
A9C00_RS25280	7352..7639	-	288	WP_000222766.1	type II toxin-antitoxin system RelE/ParE family toxin	-
A9C00_RS25285	7636..7887	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
A9C00_RS25290	8853..9710	-	858	WP_039002220.1	incFII family plasmid replication initiator RepA	-
A9C00_RS25295	9703..9777	-	75	WP_061357229.1	RepA leader peptide Tap	-
A9C00_RS25300	10009..10266	-	258	WP_001541895.1	replication regulatory protein RepA	-
A9C00_RS25305	10534..10740	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	10727..10788	+	62	NuclAT_1	-	Antitoxin
-	10727..10788	+	62	NuclAT_1	-	Antitoxin
-	10727..10788	+	62	NuclAT_1	-	Antitoxin
-	10727..10788	+	62	NuclAT_1	-	Antitoxin
A9C00_RS25310	11130..11342	-	213	WP_001541896.1	ANR family transcriptional regulator	-
A9C00_RS25315	11477..12037	-	561	WP_000139310.1	fertility inhibition protein FinO	-
A9C00_RS25320	12092..12835	-	744	WP_065304457.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	oqxB / oqxA / blaTEM-1B	-	1..129035	129035

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T63922 WP_001351576.1 NZ_CP015914:c10740-10534 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T63922 NZ_CP015914:c10740-10534 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACTGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT63922 NZ_CP015914:10727-10788 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin