Detailed information of TA system TA063913

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3034136..3034738
• Replicon: chromosome
• Accession: NZ_CP015912
• Organism: Escherichia coli strain 210205630

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: A9C00_RS15120
• Protein ID: WP_000897305.1
• Coordinates: 3034136..3034447 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A9C00_RS15125
• Protein ID: WP_000356397.1
• Coordinates: 3034448..3034738 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9C00_RS15090	3029166..3029951	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
A9C00_RS15095	3030050..3030649	+	600	WP_001315111.1	glucose-1-phosphatase	-
A9C00_RS15100	3030643..3031515	+	873	WP_000920762.1	virulence factor BrkB family protein	-
A9C00_RS15105	3031512..3031949	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
A9C00_RS15110	3031994..3032935	+	942	WP_063104924.1	fatty acid biosynthesis protein FabY	-
A9C00_RS15115	3032999..3033907	-	909	WP_065304282.1	alpha/beta hydrolase	-
A9C00_RS15120	3034136..3034447	+	312	WP_000897305.1	hypothetical protein	Toxin
A9C00_RS15125	3034448..3034738	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
A9C00_RS15130	3035343..3035561	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
A9C00_RS15135	3035780..3036022	+	243	WP_001086388.1	hypothetical protein	-
A9C00_RS15145	3036352..3037281	-	930	WP_001749031.1	formate dehydrogenase accessory protein FdhE	-
A9C00_RS15150	3037278..3037913	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
A9C00_RS15155	3037910..3038812	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T63913 WP_000897305.1 NZ_CP015912:3034136-3034447 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T63913 NZ_CP015912:3034136-3034447 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT63913 WP_000356397.1 NZ_CP015912:3034448-3034738 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT63913 NZ_CP015912:3034448-3034738 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7