Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 973039..973541 Replicon chromosome
Accession NZ_CP015912
Organism Escherichia coli strain 210205630

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag A9C00_RS05005 Protein ID WP_000767829.1
Coordinates 973039..973293 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag A9C00_RS05010 Protein ID WP_001259255.1
Coordinates 973290..973541 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9C00_RS04980 968054..968281 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
A9C00_RS04985 968295..969353 - 1059 WP_000492324.1 transport protein YeeE -
A9C00_RS04990 969532..970890 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
A9C00_RS30150 970880..970942 - 63 WP_010723108.1 membrane protein YoeI -
A9C00_RS04995 971157..972086 - 930 WP_000803351.1 LysR family transcriptional regulator -
A9C00_RS05000 972132..972956 - 825 WP_000754737.1 SDR family oxidoreductase -
A9C00_RS05005 973039..973293 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
A9C00_RS05010 973290..973541 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
A9C00_RS27550 973824..973874 + 51 WP_001364200.1 his operon leader peptide -
A9C00_RS05015 974020..974919 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
A9C00_RS05020 974925..976229 + 1305 WP_001345878.1 histidinol dehydrogenase -
A9C00_RS05025 976226..977296 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
A9C00_RS05030 977296..978363 + 1068 WP_000080109.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T63906 WP_000767829.1 NZ_CP015912:c973293-973039 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T63906 NZ_CP015912:c973293-973039 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT63906 WP_001259255.1 NZ_CP015912:c973541-973290 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT63906 NZ_CP015912:c973541-973290 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References