Detailed information of TA system TA063841

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3083873..3084486
• Replicon: chromosome
• Accession: NZ_CP015878
• Organism: Pseudomonas citronellolis strain SJTE-3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1A9KC21
• Locus tag: A9C11_RS13800
• Protein ID: WP_064582966.1
• Coordinates: 3083873..3084169 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A9C11_RS13805
• Protein ID: WP_024128404.1
• Coordinates: 3084178..3084486 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9C11_RS13790	3081084..3082250	-	1167	WP_064582963.1	efflux RND transporter periplasmic adaptor subunit	-
A9C11_RS13795	3082247..3083650	-	1404	WP_064582964.1	efflux transporter outer membrane subunit	-
A9C11_RS13800	3083873..3084169	+	297	WP_064582966.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A9C11_RS13805	3084178..3084486	+	309	WP_024128404.1	HigA family addiction module antidote protein	Antitoxin
A9C11_RS13810	3084693..3085400	-	708	WP_064582968.1	response regulator transcription factor	-
A9C11_RS13815	3085505..3086194	+	690	WP_058489774.1	response regulator transcription factor	-
A9C11_RS13820	3086172..3087551	+	1380	WP_064582969.1	HAMP domain-containing protein	-
A9C11_RS13825	3087737..3088309	-	573	WP_064582970.1	flavin reductase family protein	-
A9C11_RS13830	3088326..3089246	-	921	WP_009619989.1	bestrophin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11760.26 Da
• Isoelectric Point: 8.0917

>T63841 WP_064582966.1 NZ_CP015878:3083873-3084169 [Pseudomonas citronellolis]
MIGSFRDAWLEAFFVRDRMPRQIPADIQERLFRKLQMLDDATTDADLRSPPSNHFERLQGRLRAYHSIRVNRQWRLVFHW
DGEKGEASDVCLDNHSYR

Nucleotide

• Length: 297 bp

>T63841 NZ_CP015878:3083873-3084169 [Pseudomonas citronellolis]
ATGATCGGGAGTTTCAGGGATGCGTGGCTGGAAGCGTTCTTCGTGCGGGACAGGATGCCCCGGCAGATTCCCGCGGATAT
CCAGGAGCGCCTGTTCCGCAAGCTGCAGATGCTCGACGACGCCACCACGGACGCGGACTTGCGTAGCCCACCCAGCAACC
ATTTCGAGCGCTTGCAGGGGCGCCTGCGGGCATACCACTCGATCCGGGTGAATCGCCAATGGCGCCTGGTCTTTCATTGG
GATGGCGAAAAAGGCGAAGCCTCGGACGTCTGCCTCGACAACCATTCGTACCGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11471.20 Da
• Isoelectric Point: 11.1591

>AT63841 WP_024128404.1 NZ_CP015878:3084178-3084486 [Pseudomonas citronellolis]
VIITERKPVSVGEVLSSEFLDPLGLTQGRLAEAMGVQRKLVNELCNDRRAVTADTALMLARVFGNSAEFWLNLQRRNDLW
AALNSPERRARIERARPLNRAA

Nucleotide

• Length: 309 bp

>AT63841 NZ_CP015878:3084178-3084486 [Pseudomonas citronellolis]
GTGATCATCACCGAACGCAAGCCGGTCAGCGTCGGCGAGGTGCTCAGCAGCGAGTTCCTCGATCCGCTAGGCCTGACCCA
AGGCCGCCTCGCCGAAGCGATGGGGGTGCAGCGCAAGCTGGTCAACGAACTGTGCAACGACCGGAGGGCAGTGACGGCCG
ATACCGCCCTGATGCTGGCCCGGGTATTCGGCAACTCCGCGGAGTTCTGGCTCAACCTGCAGCGGCGCAACGACCTGTGG
GCGGCGTTGAATTCGCCAGAGCGACGTGCCCGCATAGAGCGGGCTCGGCCATTGAATCGGGCGGCCTGA

Structures

Toxin

• AlphaFold DB: A0A1A9KC21