Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 3054747..3055369 Replicon chromosome
Accession NZ_CP015878
Organism Pseudomonas citronellolis strain SJTE-3

Toxin (Protein)


Gene name higB Uniprot ID A0A127MSF6
Locus tag A9C11_RS13665 Protein ID WP_043317089.1
Coordinates 3055055..3055369 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1A9KBU6
Locus tag A9C11_RS13660 Protein ID WP_064582938.1
Coordinates 3054747..3055052 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9C11_RS13630 3050019..3050228 - 210 WP_009619863.1 DUF2945 domain-containing protein -
A9C11_RS13635 3050425..3051810 + 1386 WP_009619865.1 oxygen-independent coproporphyrinogen III oxidase -
A9C11_RS13640 3051905..3052753 - 849 WP_064582937.1 amidohydrolase family protein -
A9C11_RS13650 3053500..3053877 - 378 WP_082936921.1 integrase -
A9C11_RS36080 3054184..3054459 - 276 WP_208858266.1 hypothetical protein -
A9C11_RS13660 3054747..3055052 - 306 WP_064582938.1 helix-turn-helix domain-containing protein Antitoxin
A9C11_RS13665 3055055..3055369 - 315 WP_043317089.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A9C11_RS34135 3055449..3055631 - 183 WP_082936745.1 hypothetical protein -
A9C11_RS13675 3057926..3058276 - 351 WP_009619928.1 hypothetical protein -
A9C11_RS13680 3058708..3059133 + 426 WP_058489395.1 hypothetical protein -
A9C11_RS13685 3059439..3059858 - 420 WP_064582942.1 LytTR family transcriptional regulator DNA-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3022061..3059133 37072


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(43-101)

Antitoxin

Cro/C1-type helix-turn-helix domain

(36-72)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11744.48 Da        Isoelectric Point: 9.4385

>T63840 WP_043317089.1 NZ_CP015878:c3055369-3055055 [Pseudomonas citronellolis]
MIFIETPVFTKRILALVDDETYRKLQEDLTLHPDAGAVIEGTGGVRKIRIAANGHGKRGGARVIYYHFTSASQIAFLLVY
DKASQEDLTADQKKVLRQIVENWR

Download         Length: 315 bp

>T63840 NZ_CP015878:c3055369-3055055 [Pseudomonas citronellolis]
ATGATCTTCATCGAAACGCCAGTCTTCACGAAGCGGATTCTTGCGCTAGTAGACGATGAGACCTATCGCAAGCTTCAGGA
AGACCTAACGCTCCACCCGGACGCCGGAGCAGTCATTGAAGGCACGGGTGGCGTGCGCAAGATTCGCATTGCTGCCAATG
GCCACGGGAAAAGAGGCGGCGCCCGGGTCATCTACTACCACTTCACATCCGCCTCTCAAATTGCATTCCTGCTGGTCTAT
GACAAGGCATCGCAGGAGGACCTGACTGCCGATCAGAAAAAGGTACTTCGGCAGATCGTTGAGAACTGGAGATAG

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11357.85 Da        Isoelectric Point: 7.2080

>AT63840 WP_064582938.1 NZ_CP015878:c3055052-3054747 [Pseudomonas citronellolis]
MDKELFAELVESATQMDEIVRGERQPSREFHIDAVKVREIRRATGLSQAKFARKINVAVGTLRNWEQGRRDPEGPARALL
RAIDNDPVHVLAALNGGPRED

Download         Length: 306 bp

>AT63840 NZ_CP015878:c3055052-3054747 [Pseudomonas citronellolis]
ATGGACAAAGAACTCTTCGCTGAACTGGTCGAAAGCGCGACCCAGATGGACGAGATCGTGCGCGGCGAACGTCAACCTTC
CCGCGAATTCCACATCGACGCCGTGAAGGTTCGCGAAATCCGCCGCGCTACTGGCCTGTCCCAGGCCAAATTCGCCCGGA
AAATTAATGTTGCTGTCGGCACGCTGCGCAATTGGGAGCAAGGGCGCCGCGATCCCGAGGGGCCTGCTCGCGCGCTGCTC
CGAGCCATCGATAATGATCCGGTCCATGTTCTAGCTGCCCTGAATGGCGGCCCGCGAGAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A127MSF6

Antitoxin

Source ID Structure
AlphaFold DB A0A1A9KBU6

References