Detailed information of TA system TA063830

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2666625..2667306
• Replicon: chromosome
• Accession: NZ_CP015877
• Organism: Pseudomonas aeruginosa SJTD-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: A214_RS12285
• Protein ID: WP_015503432.1
• Coordinates: 2666625..2666990 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A214_RS12290
• Protein ID: WP_016561576.1
• Coordinates: 2666983..2667306 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A214_RS12255	2662630..2663064	-	435	WP_016253806.1	RidA family protein	-
A214_RS12260	2663285..2664238	+	954	WP_003085661.1	LysR family transcriptional regulator	-
A214_RS12265	2664211..2665095	-	885	WP_003454589.1	LysR family transcriptional regulator	-
A214_RS12270	2665196..2665621	+	426	WP_003114206.1	ring-cleaving dioxygenase	-
A214_RS12275	2665640..2665912	-	273	WP_003085667.1	hypothetical protein	-
A214_RS12280	2666119..2666361	+	243	WP_043884955.1	hypothetical protein	-
A214_RS12285	2666625..2666990	+	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A214_RS12290	2666983..2667306	+	324	WP_016561576.1	XRE family transcriptional regulator	Antitoxin
A214_RS29565	2667422..2667541	-	120	Protein_2418	IS5/IS1182 family transposase	-
A214_RS12295	2667596..2667928	+	333	WP_016561578.1	hypothetical protein	-
A214_RS28845	2667928..2668203	+	276	WP_004353146.1	hypothetical protein	-
A214_RS28850	2668378..2668920	+	543	WP_019396018.1	tyrosine-type recombinase/integrase	-
A214_RS28855	2668935..2669162	-	228	WP_014603215.1	hypothetical protein	-
A214_RS12305	2670173..2670721	+	549	WP_064612271.1	3'-5' exonuclease	-
A214_RS28860	2670718..2671209	-	492	WP_071536159.1	Mov34/MPN/PAD-1 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T63830 WP_015503432.1 NZ_CP015877:2666625-2666990 [Pseudomonas aeruginosa SJTD-1]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T63830 NZ_CP015877:2666625-2666990 [Pseudomonas aeruginosa SJTD-1]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCCTG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11903.72 Da
• Isoelectric Point: 5.8900

>AT63830 WP_016561576.1 NZ_CP015877:2666983-2667306 [Pseudomonas aeruginosa SJTD-1]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQSRGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT63830 NZ_CP015877:2666983-2667306 [Pseudomonas aeruginosa SJTD-1]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGTCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCAGGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATTGCACGCTTCCCGGATGGTGCGGTGAAAATCAGCAACTTCGCGGATATCGAAGAGGCGGGCGC
CTAA