Detailed information of TA system TA063825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 1807341..1808041
• Replicon: chromosome
• Accession: NZ_CP015876
• Organism: Pseudomonas putida SJTE-1

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: Q88F93
• Locus tag: A210_RS08250
• Protein ID: WP_010954960.1
• Coordinates: 1807341..1807637 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: I7AUJ7
• Locus tag: A210_RS08255
• Protein ID: WP_003254235.1
• Coordinates: 1807640..1808041 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A210_RS08230	1802977..1804602	+	1626	WP_017148195.1	twin-arginine translocation signal domain-containing protein	-
A210_RS08235	1804724..1805200	+	477	WP_012051715.1	sigma-70 family RNA polymerase sigma factor	-
A210_RS08240	1805369..1805848	+	480	WP_012051716.1	hypothetical protein	-
A210_RS08245	1805921..1807246	+	1326	WP_012051717.1	MFS transporter	-
A210_RS08250	1807341..1807637	+	297	WP_010954960.1	type II toxin-antitoxin system MqsR family toxin	Toxin
A210_RS08255	1807640..1808041	+	402	WP_003254235.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
A210_RS08260	1808113..1809795	-	1683	WP_012051718.1	electron transfer flavoprotein-ubiquinone oxidoreductase	-
A210_RS08265	1810331..1811080	+	750	WP_014755872.1	electron transfer flavoprotein subunit beta/FixA family protein	-
A210_RS08270	1811081..1812010	+	930	WP_014755873.1	FAD-binding protein	-
A210_RS08275	1812086..1812907	+	822	WP_012051719.1	transporter substrate-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (6-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (74-109)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11133.22 Da
• Isoelectric Point: 10.2271

>T63825 WP_010954960.1 NZ_CP015876:1807341-1807637 [Pseudomonas putida SJTE-1]
MEKRMPHCPLERVKALAAARRIRPTGAALKGAKALGMDFPGMLEVITSLKRTDFYKSMTSHIDHRVWQDVYRPLTAIGYV
YLKLSVVDDVLIVSFKEL

Nucleotide

• Length: 297 bp

>T63825 NZ_CP015876:1807341-1807637 [Pseudomonas putida SJTE-1]
ATGGAAAAGAGAATGCCTCATTGCCCGCTGGAGCGGGTAAAAGCCCTGGCTGCGGCAAGGCGAATCCGCCCTACGGGTGC
TGCATTGAAAGGCGCCAAGGCGCTGGGAATGGATTTTCCGGGCATGCTTGAGGTGATCACCAGCTTGAAGCGCACTGATT
TCTACAAGAGCATGACCAGCCATATTGACCACCGGGTTTGGCAGGATGTGTATCGCCCGCTTACAGCAATTGGGTACGTT
TATTTGAAATTGTCCGTGGTAGACGACGTGCTCATCGTGTCTTTCAAGGAGTTGTAA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14684.76 Da
• Isoelectric Point: 4.9531

>AT63825 WP_003254235.1 NZ_CP015876:1807640-1808041 [Pseudomonas putida SJTE-1]
MRCPICGGSELAPDIQGMPYSYKGEMTVIPEVSGDYCSACGECVLSHDEAMRVSHLMTAFERQVNANVVDPSFIASIRRK
FDLDQREAGEIFGGGVNAFSRYENGKTTPPVALVKLLKLLDRHPELFEEVRTA

Nucleotide

• Length: 402 bp

>AT63825 NZ_CP015876:1807640-1808041 [Pseudomonas putida SJTE-1]
ATGAGATGTCCAATATGCGGCGGCTCGGAGCTTGCACCTGACATTCAGGGCATGCCCTATAGCTACAAAGGCGAGATGAC
AGTGATCCCTGAGGTAAGTGGCGATTACTGCTCCGCATGTGGTGAATGTGTACTGAGTCATGATGAAGCCATGCGCGTCA
GTCACTTGATGACGGCATTCGAGCGCCAGGTCAACGCAAATGTTGTGGATCCTTCCTTTATTGCCTCCATACGCAGAAAG
TTCGACCTCGACCAGCGTGAAGCGGGGGAAATCTTCGGTGGTGGGGTCAATGCGTTCTCCCGTTATGAAAACGGCAAGAC
CACACCGCCAGTGGCGTTGGTGAAACTGCTCAAGCTGCTGGATCGCCATCCAGAACTCTTTGAGGAAGTGCGTACTGCCT
GA

Structures

Toxin

• AlphaFold DB: Q88F93

Antitoxin

• AlphaFold DB: I7AUJ7