Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2411877..2412416 Replicon chromosome
Accession NZ_CP015853
Organism Escherichia coli strain ATCC 43889

Toxin (Protein)


Gene name yafQ Uniprot ID -
Locus tag A8714_RS12870 Protein ID WP_001303998.1
Coordinates 2412156..2412416 (+) Length 87 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID C3TP07
Locus tag A8714_RS12865 Protein ID WP_000729705.1
Coordinates 2411877..2412137 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A8714_RS12845 2407631..2408416 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
A8714_RS12850 2408388..2410100 + 1713 Protein_2424 flagellar biosynthesis protein FlhA -
A8714_RS12855 2410245..2410742 - 498 Protein_2425 REP-associated tyrosine transposase RayT -
A8714_RS12860 2410918..2411691 - 774 WP_000543891.1 C40 family peptidase -
A8714_RS12865 2411877..2412137 + 261 WP_000729705.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
A8714_RS12870 2412156..2412416 + 261 WP_001303998.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
A8714_RS12875 2412572..2413312 + 741 WP_001225679.1 murein L,D-transpeptidase -
A8714_RS12880 2413283..2414050 - 768 WP_001301698.1 class II glutamine amidotransferase -
A8714_RS12885 2414155..2414733 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-85)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 10026.71 Da        Isoelectric Point: 10.3188

>T63804 WP_001303998.1 NZ_CP015853:2412156-2412416 [Escherichia coli]
MNSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGT
HAALFG

Download         Length: 261 bp

>T63804 NZ_CP015853:2412156-2412416 [Escherichia coli]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9377.74 Da        Isoelectric Point: 5.0758

>AT63804 WP_000729705.1 NZ_CP015853:2411877-2412137 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Download         Length: 261 bp

>AT63804 NZ_CP015853:2411877-2412137 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References