Detailed information of TA system TA063803

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2405759..2406453
• Replicon: chromosome
• Accession: NZ_CP015853
• Organism: Escherichia coli strain ATCC 43889

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: A8714_RS12830
• Protein ID: WP_001263495.1
• Coordinates: 2405759..2406157 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: A8714_RS12835
• Protein ID: WP_000554757.1
• Coordinates: 2406160..2406453 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	2401346..2401426	-	81	NuclAT_11	-	-
-	2401346..2401426	-	81	NuclAT_11	-	-
-	2401346..2401426	-	81	NuclAT_11	-	-
-	2401346..2401426	-	81	NuclAT_11	-	-
A8714_RS12805	2402022..2402480	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
A8714_RS12810	2402741..2404198	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
A8714_RS12815	2404255..2404776	-	522	Protein_2417	peptide chain release factor H	-
A8714_RS12820	2404775..2404978	-	204	Protein_2418	RNA ligase RtcB family protein	-
A8714_RS12825	2405297..2405749	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
A8714_RS12830	2405759..2406157	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
A8714_RS12835	2406160..2406453	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
A8714_RS12840	2406505..2407560	-	1056	WP_001226186.1	DNA polymerase IV	-
A8714_RS12845	2407631..2408416	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
A8714_RS12850	2408388..2410100	+	1713	Protein_2424	flagellar biosynthesis protein FlhA	-
A8714_RS12855	2410245..2410742	-	498	Protein_2425	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T63803 WP_001263495.1 NZ_CP015853:c2406157-2405759 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T63803 NZ_CP015853:c2406157-2405759 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT63803 WP_000554757.1 NZ_CP015853:c2406453-2406160 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT63803 NZ_CP015853:c2406453-2406160 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6