Detailed information of TA system TA063744

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2209523..2209748
• Replicon: chromosome
• Accession: NZ_CP015846
• Organism: Escherichia coli O157:H7 strain FRIK2069

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: A8V30_RS11935
• Protein ID: WP_000813258.1
• Coordinates: 2209523..2209678 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2209690..2209748 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8V30_RS11885	2204526..2204957	-	432	WP_001302123.1	tellurite resistance protein	-
A8V30_RS11900	2205408..2206121	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
A8V30_RS11905	2206257..2206454	-	198	WP_000917763.1	hypothetical protein	-
A8V30_RS11910	2206679..2207233	-	555	WP_000640035.1	DUF1133 family protein	-
A8V30_RS11915	2207296..2207601	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
A8V30_RS11920	2207614..2208663	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
A8V30_RS11925	2208665..2208937	-	273	WP_000191872.1	hypothetical protein	-
A8V30_RS11930	2209059..2209403	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
A8V30_RS11935	2209523..2209678	-	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2209690..2209748	+	59	-	-	Antitoxin
A8V30_RS11940	2209969..2210526	-	558	WP_000104474.1	DUF551 domain-containing protein	-
A8V30_RS11945	2210528..2210746	-	219	WP_000683609.1	DUF4014 family protein	-
A8V30_RS11950	2210874..2211185	-	312	WP_001289673.1	hypothetical protein	-
A8V30_RS11955	2211178..2211405	-	228	WP_000699809.1	hypothetical protein	-
A8V30_RS11960	2211402..2211683	-	282	WP_000603384.1	DNA-binding protein	-
A8V30_RS11965	2211716..2212432	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
A8V30_RS31275	2212466..2212927	-	462	WP_000139447.1	replication protein	-
A8V30_RS31280	2212920..2213963	-	1044	WP_001262402.1	hypothetical protein	-
A8V30_RS11980	2214032..2214457	-	426	WP_000693878.1	Rha family transcriptional regulator	-
A8V30_RS11985	2214441..2214683	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2172411..2261669	89258

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T63744 WP_000813258.1 NZ_CP015846:c2209678-2209523 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T63744 NZ_CP015846:c2209678-2209523 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT63744 NZ_CP015846:2209690-2209748 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin