Detailed information of TA system TA063741

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1846613..1846838
• Replicon: chromosome
• Accession: NZ_CP015846
• Organism: Escherichia coli O157:H7 strain FRIK2069

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: A8V30_RS10090
• Protein ID: WP_000813254.1
• Coordinates: 1846683..1846838 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1846613..1846671 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8V30_RS10040	1841866..1842819	+	954	WP_000095667.1	helix-turn-helix domain-containing protein	-
A8V30_RS10045	1842826..1843566	+	741	WP_000790456.1	ATP-binding protein	-
A8V30_RS10050	1843596..1844366	+	771	WP_000450888.1	DUF1627 domain-containing protein	-
A8V30_RS10055	1844382..1844777	+	396	WP_001118161.1	DUF977 family protein	-
A8V30_RS10060	1844834..1845190	+	357	WP_001302146.1	hypothetical protein	-
A8V30_RS10065	1845239..1845451	+	213	WP_000063625.1	hypothetical protein	-
A8V30_RS10070	1845487..1845858	+	372	WP_000137941.1	hypothetical protein	-
A8V30_RS10075	1845855..1846217	+	363	WP_000610379.1	DUF551 domain-containing protein	-
A8V30_RS10080	1846333..1846437	+	105	WP_001278450.1	hypothetical protein	-
-	1846613..1846671	-	59	-	-	Antitoxin
A8V30_RS10090	1846683..1846838	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
A8V30_RS31720	1847135..1847302	+	168	WP_000998188.1	hypothetical protein	-
A8V30_RS10095	1847368..1847646	+	279	WP_001304183.1	hypothetical protein	-
A8V30_RS10100	1847648..1848697	+	1050	Protein_1821	DUF968 domain-containing protein	-
A8V30_RS10105	1848710..1849084	+	375	WP_000904137.1	RusA family crossover junction endodeoxyribonuclease	-
A8V30_RS10110	1849081..1849902	+	822	WP_000762904.1	antitermination protein	-
A8V30_RS10115	1850129..1850326	+	198	WP_000917735.1	hypothetical protein	-
A8V30_RS10120	1850477..1851535	+	1059	WP_000483497.1	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	paa / nleA/espI / espM1	1772901..1878915	106014

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T63741 WP_000813254.1 NZ_CP015846:1846683-1846838 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T63741 NZ_CP015846:1846683-1846838 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGCTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT63741 NZ_CP015846:c1846671-1846613 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin