Detailed information of TA system TA063736

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1279970..1280195
• Replicon: chromosome
• Accession: NZ_CP015846
• Organism: Escherichia coli O157:H7 strain FRIK2069

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: A8V30_RS06580
• Protein ID: WP_000813263.1
• Coordinates: 1280040..1280195 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1279970..1280028 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8V30_RS06555	1275245..1276336	+	1092	WP_001205823.1	hypothetical protein	-
A8V30_RS06560	1276343..1277089	+	747	WP_000788751.1	ATP-binding protein	-
A8V30_RS06565	1277111..1277881	+	771	WP_000450992.1	DUF1627 domain-containing protein	-
A8V30_RS06570	1277897..1278310	+	414	WP_001151233.1	DUF977 family protein	-
A8V30_RS06575	1278662..1279435	-	774	WP_000160654.1	alpha/beta hydrolase	-
-	1279970..1280028	-	59	-	-	Antitoxin
A8V30_RS06580	1280040..1280195	+	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
A8V30_RS06585	1280363..1280641	+	279	WP_001341388.1	hypothetical protein	-
A8V30_RS06590	1280643..1281692	+	1050	WP_001265172.1	DUF968 domain-containing protein	-
A8V30_RS06595	1281705..1282076	+	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
A8V30_RS06600	1282066..1282437	+	372	WP_000090264.1	antitermination protein	-
A8V30_RS06605	1282589..1283407	+	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
A8V30_RS06610	1283694..1283890	+	197	Protein_1197	TrmB family transcriptional regulator	-
A8V30_RS06615	1284028..1284741	+	714	WP_000261909.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T63736 WP_000813263.1 NZ_CP015846:1280040-1280195 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T63736 NZ_CP015846:1280040-1280195 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT63736 NZ_CP015846:c1280028-1279970 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin