Detailed information of TA system TA063719

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ldrD-ohsC/Ldr(toxin)
• Location: 4604284..4604505
• Replicon: chromosome
• Accession: NZ_CP015843
• Organism: Escherichia coli O157:H7 strain FRIK2455

Toxin (Protein)

• Gene name: ldrD
• Uniprot ID: S1NWX0
• Locus tag: A8V32_RS25145
• Protein ID: WP_001295224.1
• Coordinates: 4604284..4604391 (-)
• Length: 36 a.a.

Antitoxin (RNA)

Gene name	ohsC
Locus tag	-
Coordinates	4604440..4604505 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8V32_RS25120	4599537..4600289	-	753	Protein_4578	cellulose biosynthesis protein BcsQ	-
A8V32_RS25125	4600301..4600489	-	189	WP_001063316.1	YhjR family protein	-
A8V32_RS25130	4600762..4602333	+	1572	WP_001204920.1	cellulose biosynthesis protein BcsE	-
A8V32_RS25135	4602330..4602521	+	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-
A8V32_RS25140	4602518..4604197	+	1680	WP_000191592.1	cellulose biosynthesis protein BcsG	-
A8V32_RS25145	4604284..4604391	-	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	Toxin
-	4604440..4604505	+	66	NuclAT_16	-	Antitoxin
-	4604440..4604505	+	66	NuclAT_16	-	Antitoxin
-	4604440..4604505	+	66	NuclAT_16	-	Antitoxin
-	4604440..4604505	+	66	NuclAT_16	-	Antitoxin
-	4604440..4604505	+	66	NuclAT_21	-	Antitoxin
-	4604440..4604505	+	66	NuclAT_21	-	Antitoxin
-	4604440..4604505	+	66	NuclAT_21	-	Antitoxin
-	4604440..4604505	+	66	NuclAT_21	-	Antitoxin
A8V32_RS25160	4604867..4606138	+	1272	WP_001301684.1	amino acid permease	-
A8V32_RS25165	4606168..4607172	-	1005	WP_000107027.1	dipeptide ABC transporter ATP-binding subunit DppF	-
A8V32_RS25170	4607169..4608152	-	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	-
A8V32_RS25175	4608163..4609065	-	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type I toxin-antitoxin system, toxin Ldr (1-35)

Sequences

Toxin

Protein

• Length: 36 a.a.
• Molecular weight: 3882.70 Da
• Isoelectric Point: 9.0157

>T63719 WP_001295224.1 NZ_CP015843:c4604391-4604284 [Escherichia coli O157:H7]
MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK

Nucleotide

• Length: 108 bp

>T63719 NZ_CP015843:c4604391-4604284 [Escherichia coli O157:H7]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT63719 NZ_CP015843:4604440-4604505 [Escherichia coli O157:H7]
GTCTAGGTTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A4P7TSJ7

Antitoxin