Detailed information of TA system TA063697

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1846616..1846841
• Replicon: chromosome
• Accession: NZ_CP015843
• Organism: Escherichia coli O157:H7 strain FRIK2455

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: A8V32_RS10090
• Protein ID: WP_000813254.1
• Coordinates: 1846686..1846841 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1846616..1846674 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8V32_RS10040	1841869..1842822	+	954	WP_000095667.1	helix-turn-helix domain-containing protein	-
A8V32_RS10045	1842829..1843569	+	741	WP_000790456.1	ATP-binding protein	-
A8V32_RS10050	1843599..1844369	+	771	WP_000450888.1	DUF1627 domain-containing protein	-
A8V32_RS10055	1844385..1844780	+	396	WP_001118161.1	DUF977 family protein	-
A8V32_RS10060	1844837..1845193	+	357	WP_001302146.1	hypothetical protein	-
A8V32_RS10065	1845242..1845454	+	213	WP_000063625.1	hypothetical protein	-
A8V32_RS10070	1845490..1845861	+	372	WP_000137941.1	hypothetical protein	-
A8V32_RS10075	1845858..1846220	+	363	WP_000610379.1	DUF551 domain-containing protein	-
A8V32_RS10080	1846336..1846440	+	105	WP_001278450.1	hypothetical protein	-
-	1846616..1846674	-	59	-	-	Antitoxin
A8V32_RS10090	1846686..1846841	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
A8V32_RS31965	1847138..1847305	+	168	WP_000998188.1	hypothetical protein	-
A8V32_RS10095	1847371..1847649	+	279	WP_001304183.1	hypothetical protein	-
A8V32_RS10100	1847651..1848700	+	1050	Protein_1824	DUF968 domain-containing protein	-
A8V32_RS10105	1848713..1849087	+	375	WP_000904137.1	RusA family crossover junction endodeoxyribonuclease	-
A8V32_RS10110	1849084..1849905	+	822	WP_000762904.1	antitermination protein	-
A8V32_RS10115	1850132..1850329	+	198	WP_000917735.1	hypothetical protein	-
A8V32_RS10120	1850480..1851538	+	1059	WP_000483497.1	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	paa / nleA/espI / espM1	1772908..1889750	116842

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T63697 WP_000813254.1 NZ_CP015843:1846686-1846841 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T63697 NZ_CP015843:1846686-1846841 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGCTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT63697 NZ_CP015843:c1846674-1846616 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin