Detailed information of TA system TA063692

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1279980..1280205
• Replicon: chromosome
• Accession: NZ_CP015843
• Organism: Escherichia coli O157:H7 strain FRIK2455

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: A8V32_RS06575
• Protein ID: WP_000813263.1
• Coordinates: 1280050..1280205 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1279980..1280038 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8V32_RS06550	1275255..1276346	+	1092	WP_001205823.1	hypothetical protein	-
A8V32_RS06555	1276353..1277099	+	747	WP_000788751.1	ATP-binding protein	-
A8V32_RS06560	1277121..1277891	+	771	WP_000450992.1	DUF1627 domain-containing protein	-
A8V32_RS06565	1277907..1278320	+	414	WP_001151233.1	DUF977 family protein	-
A8V32_RS06570	1278672..1279445	-	774	WP_000160654.1	alpha/beta hydrolase	-
-	1279980..1280038	-	59	-	-	Antitoxin
A8V32_RS06575	1280050..1280205	+	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
A8V32_RS06580	1280373..1280651	+	279	WP_001341388.1	hypothetical protein	-
A8V32_RS06585	1280653..1281702	+	1050	WP_001265172.1	DUF968 domain-containing protein	-
A8V32_RS06590	1281715..1282086	+	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
A8V32_RS06595	1282076..1282447	+	372	WP_000090264.1	antitermination protein	-
A8V32_RS06600	1282599..1283417	+	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
A8V32_RS06605	1283704..1283900	+	197	Protein_1196	TrmB family transcriptional regulator	-
A8V32_RS06610	1284038..1284751	+	714	WP_000261909.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T63692 WP_000813263.1 NZ_CP015843:1280050-1280205 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T63692 NZ_CP015843:1280050-1280205 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT63692 NZ_CP015843:c1280038-1279980 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin