Detailed information of TA system TA063687

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 285672..286366
• Replicon: chromosome
• Accession: NZ_CP015843
• Organism: Escherichia coli O157:H7 strain FRIK2455

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: A8V32_RS01365
• Protein ID: WP_001263495.1
• Coordinates: 285968..286366 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: A8V32_RS01360
• Protein ID: WP_000554757.1
• Coordinates: 285672..285965 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8V32_RS01340	281383..281880	+	498	Protein_247	REP-associated tyrosine transposase RayT	-
A8V32_RS01345	282025..283737	-	1713	Protein_248	flagellar biosynthesis protein FlhA	-
A8V32_RS01350	283709..284494	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
A8V32_RS01355	284565..285620	+	1056	WP_001226186.1	DNA polymerase IV	-
A8V32_RS01360	285672..285965	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
A8V32_RS01365	285968..286366	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
A8V32_RS01370	286376..286828	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
A8V32_RS01375	287147..287350	+	204	Protein_254	RNA ligase RtcB family protein	-
A8V32_RS01380	287349..287870	+	522	Protein_255	peptide chain release factor H	-
A8V32_RS01385	287927..289384	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
A8V32_RS01390	289645..290103	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	290699..290779	+	81	NuclAT_11	-	-
-	290699..290779	+	81	NuclAT_11	-	-
-	290699..290779	+	81	NuclAT_11	-	-
-	290699..290779	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T63687 WP_001263495.1 NZ_CP015843:285968-286366 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T63687 NZ_CP015843:285968-286366 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT63687 WP_000554757.1 NZ_CP015843:285672-285965 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT63687 NZ_CP015843:285672-285965 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6